Common Bacterial ID Questions Flashcards
Gram -, comma shaped, commonly found in fecal material.
Campylobacter (Jejuni)
Gram -, comma shaped, grows best between 37–42 °C
Campylobacter (Jejuni)
Campylobacter (Jejuni) grows best at what temp range, and on what media?
They grow best between 37–42 °C in a microaerophilic environment. Blood agar (like Skirrow, Butzler, and Campy-BAP).
Campylobacter
- Gram status
- shape
- motility
- oxidase test result
- catalase test result
- Gram - tive
- comma / corkscrew shaped,
- flagellated (unipolar or bipolar flagella)
- oxidase +ve
- catalase +ve
Campylobacter (Jejuni) symptomatology includes:
- -
- fever, muscle pain, malaise and headache
- gastroenteritis
- cramping & abdominal pain,
- foul smelling diarrhea
- bloody diarrhea
How is Campylobacter (Jejuni) normally detected / sampled?
Laboratory culturing a
- stool sample or
- rectal swab
Bordetella pertussis
- Gram status
- shape
- motility
- oxidase test result
- catalase test result
- Gram -tive
- coccobacilli
- non-motile
- weakly oxidase positive
- catalase test result
Bordetella pertussis
- Gram status
- shape
- motility
- oxidase test result
- catalase test result
- Gram -ve
- coccobacilli
- non-motile
- weakly oxidase +ve
- catalase +ve
Bordetella pertussis
They are capsule +ve, catalase +ve, citrate -ve, gram -ve, non-motile, oxidase +ve, urease -ve
Does Bordetella pertussis possess a capsule?
Yes.
Bordetella pertussis is capsule +ve
What organism is displayed in this image?
Campylobacter
Comma shaped, cork screw appearance, flagellated.
Name six (six) microbiotic organisms
which contain/employ a polysaccharide capsule.
1) Streptococcus pneumoniae
2) Klebsiella pneumoniae
3) Haemophilus Influenzae
4) Pseudomonas aeruginosa
5) Neisseria (meningitidis and gonorrhoehae)
6) Cryptococcus neoformans
The 3 basic structures of the prokaryotic flagellum are what?
1) filament (flagellin)
2) hook (rotates to rotate filament)
3) basal body (anchors the filamengt and hook within the cell wall)
Flagellar structures differ between prokaryotic and eukaryotic cells.
A cell with a singular flagellum at one end is refered to as…
monotrichous
A cell with more then one flagellum, but only at one end is refered to as…
lophotrichous
A cell with more then one flagellum, located at the poles of the orgnaism are refered to as…
amphitrichous
A cell with multiple flagellum distributed about the surface of the organism are refered to as…
peritrichous
The main function of fimbriae is…
attachment.
Allows bacteria to attach to a surface.
Bacterial pili serve several functions including
1) user to transfer genetic information through conjugation.
2) attachmeent to surface
3) may assist in motility
The nucleoid within a bacteria generally contains a double stranded DNA strand that carries essential genes. Non-essential genes are commonly located where?
within a plasmid
Plasmids may be copied at any time.
Chromosomes are only copied during cell division.
Prokaryotic cells contain non-membrane bound ribosomes.
What is the weight of the prokaryotic ribosome?
70S
Prokaryotic cells contain non-membrane bound ribosomes.
What are the weights of prokaryotic ribosomal subunits.
Large subunit = 50S
Small subunit = 30S
Combined they are 70S
