Coding (DNA)

Question 1

BLAST

Answer 1

Basic
Local
Alignment

Question 2

BlastN

Answer 2

• where N is for Nucleotide
• will search all available nucleotide (DNA) seqs in GenBank for matches to your seq

Question 3

CLUSTALW

Answer 3

• multiple seq alignment programme that can align no. of DNA or protein seqs
• will identify regions that are…
  -> absolutely conserved (a.a / nucleotides are identical)
  -> relatively conserved (a.a / nucleotides are not identical, but are similar types),
  -> not conserved
• identification of conserved seq regions can be very useful in predicting structure & function of genes / proteins & in identifying new members of protein families