CMB2000/L15 Genomics & Genome Projects

Question 1

Give 2 revelations from analyses of mutant Msh2 proteins.

Answer 1

Defects in critical protein-protein interactions
Reduced steady state levels of Msh2
Mutations affected activity of mismatch repair complex

Question 2

Which procedure allowed analysis of predicted catalytic mutant Msh2 proteins from human colon cancer?

Answer 2

Expression of the proteins in yeast

Question 3

Give 3 other benefits of studies in model organisms.

Answer 3

Genetic variation
Bottleneck in personalised medicine
Mutation effects
Potential treatments

Question 4

Give a protein analysis programme.

Answer 4

PSORTII

Question 5

Where does Yox1 localise in S. pombe and when?

Answer 5

Nucleus
All stages of cell cycle

Question 6

Give a protein domain identification programme.

Answer 6

BLAST

Question 7

Define a homeodomain.

Answer 7

DNA binding domains involved in transcriptional regulation of key automatic processes
May bind to DNA as monomers or as homo- or heterodimers in a sequence-specific manner

Question 8

Give a phosphorylation site analysis programme.

Answer 8

NetPhos

Question 9

Give 3 approaches to phosphorylation analysis.

Answer 9

Investigate protein phosphorylation in vivo and whether residue is important
Test genetically and biochemically potential role of programme predicted kinase
Whether phosphorylation changes in cell cycle-dependent manner
Mutate residue to investigate role of phosphorylation

Question 10

What is the transcription factor DNA binding sequence?

Answer 10

TATTTACGCG