CM Flashcards
Required urine volume for drug testing
35-45 mL
Container capacity for drug testing
60 mL
Temp of urine for drug testing and checked within how many minutes
32.5-37.7 C ; within 4 minutes
Increased in unpreserved urine
pH
Bacteria
Odor
Nitrite
(Pa BaON)
Urine preservative that does not interfere with drug & hormone analyses ; preserves protein & formed elements well
Boric acid
Preservative of choice for Addis Count
Formaldehyde/ formalin
Used for urine sediment preservation
Formalin/formaldehyde
Best all-around preservative
Toluene
Urine preservative that prevents glycolysis
Sodium flouride
Urine preservative used for cytological studies
Saccomanno fixative (50% ethanol + 2% carbowax)
Normal urine volume (24 hrs)
600 to 2000 mL
Ave urine vol (24 hrs)
1,200 to 1,500 mL
Required urine vol for routine UA
10 to 15 mL
Complete cessation of urine
Anuria
Volume of urine to be considered as anuria
<100 mL/ 24 hrs
Urine mL to consider polyuria
> 2,000 mL/24 hrs
Decreased urine vol ( <400mL or <500mL/24 hrs)
Oliguria
Reagent strip with 60 sec reactions
Protein
Blood
Urobilinogen
Nitrite
Protein rgt strip principle
Protein error of indicators
Protein rgt strip reagents
Multistix: TETRABROMphenol blue, citrate buffer @ pH 3.0
Chemstrip: TETRAchlorophenol TETRABROMosulfonphthalein, citrate buffer @ pH 3.0
a.k.a Exton’s test
Sulfosalicylic acid precipitation test (SSA)
SSA grading: distinct turbidity with no granulation
1+
30-50 mg/dL or approx 50 mg/dL
SSA grading: turbidity with granulation but NO flocculation
2+
100-200 mg/dL
SSA grading: turbidity with granulation AND flocculation
3+
200-400 mg/dL
SSA grading: clumps of protein
4+
>400 mg/dL
2 procedures of SSA test
3 mL of 3% SSA + 3mL urine —— 10 min incub —— (+) cloudiness [3^3]
3 mL of 7% SSA + 11mL urine —— 10 min incub —— (+) cloudiness [3 7/11]
Rgt strip principle for glucose
Double sequential enzyme rxn
Glucose rgt strip reagents
Multistix: Glucose oxidase, Peroxidase, K iodide (blue to green to brown)
Chemstrip: Glucose oxidase, Peroxidase, Tetramethylbenzidine (yellow to green)
Rgt strip for ketones principle (40 sec)
Sodium nitroprusside (Legal’s test)
Composition of acetest tablet for ketones
Na nitroprusside, disodium phosphate, glycine, lactose
Cloudy red urine vs
Clear red urine vs
Clear red-brown urine
Hematuria
Hemoglobinuria
Myoglobinuria
Principle of rgt strip for blood (60 sec)
Pseudoperoxidase activity of hemoglobin
Rgt strip for blood reagents
‘Di’……………..’benzidine’
Basta yung mahaba na may “Di” sa unahan at “benzidine” sa dulo
Rgt strip reagents of bilirubin
Multistix: 2,4-DICHLOROaniline diazonium salt
Chemstrip: 2,6-DICHLORObensene diazonium salt