Clinical Microbiology Flashcards
to learn different procedures in clinics, and different methods used to obtain samples
The following agents should never be cultured in-house
Brucella abortus
Francisella tularensis
Yersinia pestis
Histoplasma capsulatum
Blastomyces dermatididis
Samples most commonly collected for culture include…
blood, feces, urine, washes of infected areas
T/F: when collecting a sample from an abscess via swabbing, you should only collect purulent material
False: when swabbing an abscess, it is generally better to collect samples from the leading edge or capsule of the abscess
What is important to note about milk when it is being shipped?
it can be frozen
organisms most commonly sought from feces
Salmonella, Campylobacter, Clostridium perfringes, Clostridium difficile, and Mycobacterium
what is gram stain used for?
to determine if a bacteria is gram+ or gram-
what is acid-fast stain used for?
to determine if a bacteria has endospores present
Aerobic
with oxygen
Anaerobic
without oxygen
Bacteremia or an infection of the blood is also known as?
sepsis
What medium is used for joint fluid cultures?
blood cultures/blood agar
what is the most common yeast found in ears of patients with otitis externa?
Malassezia pachydermatis
What anticoagulant prevents bacterial growth?
EDTA
T/F: it is not important to correctly label all samples with patient’s name and collection date
False: you should ALWAYS correctly label samples with the patients name and collection date
__________ is the preferred collection method for bacterial culture because of presence of indigenous flora of external genitalia
cystocentesis
local infections that spread to multiple organs are called…
disseminated or systemic infections
infection of the blood is termed as?
bacteremia
when do you perform a blood culture in a patient with bacteremia?
when systemic disease is suspected
T/F: CMT helps to confirm diagnosis of clinical mastitis
False: CMT helps to diagnose subclinical mastitis
Malassezia pachydermatis can also be seen in patients with…
dermatitis, particularly patients with underlying diseases that can cause immunosuppressin such as diabetes mellitus and hypothyroidism
what fungal organism is associated with vaginitis?
Candida albicans
what dimorphic fungus is typically found in the San Joaquin Valley in California?
Coccidioides immitis
why is Coccidioides immitis relevant to us in TN?
because of the heavy population of people moving here from California
Cryptococcus neoformans can be identified by?
abundant capsular material surrounding a yeast cell
what is a common infection/disease associated with Cryptococcus neoformans?
rhinitis
a dimorphic fungus diagnosed by visualization of yeast in the tissues or fluids from exudative lesions
Histoplasma capsulatum
Histoplasma capsulatum lives where?
in the soil/leaves
T/F: Histoplasma capsulatum has zoonotic potential
True
a dimorphic fungus diagnosed by cytological examination of prepared patient specimens
Blastomyces dermatididis (“Blasto”)
where does Blastomyces dermatididis live?
in the soil/leaves
T/F: “Blasto” has zoonotic potential
True
dogs infected with “Blasto” can show these clinical signs…..
dyspnea, cutaneous lesions, anorexia, and lameness with osteomyelitis
A dimorphic fungus that grows on decaying plant material, causing cutaneous and disseminated lesions in cats and humans
Sporothrix schenckii
T/F: Sporothrix schenckii has zoonotic potential
True
Sporothrix schenckii lives where?
decaying plant material
