CLEARING

Question 1

Should be miscible with alcohol to remove ______ from the tissue

Answer 1

dehydrating agent

Question 2

Generally, clearing agent should be miscible with ________ to facilitate embedding

Answer 2

paraffin wax

Question 3

should be miscible with ________ and other resins for during mounting, this is also the mounting media

Answer 3

Canada balsam

Question 4

What should the tissue look like after staining?

Answer 4

transparent

Question 5

most clearing agents are _______ that requires proper handling

Answer 5

flammable liquids

Question 6

it affects speed of penetration of the clearing agent

Answer 6

Viscosity

Question 7

to most clearing agents causes tissue to become brittle and more difficult to cut

Answer 7

prolonged exposure

Question 8

What are the carcinogenic clearing agents?

Answer 8

1. Xylene/xylol
2. Benzene

Question 9

What is the only non-carcinogenic clearing agent?

Answer 9

Toluene

Question 10

What clearing agent is:
-most commonly used in routine procedures
-colorless clearinga gent
-turns MILKY when incompletely dehydrated

Answer 10

Xylene/Xylol

Question 11

What is the clearing time for xylene/xylol?

Answer 11

30-60 minutes

Question 12

What are the non-flammable clearing agents?

Answer 12

1. Chloroform
2. Carbon tetrachloride

Question 13

What are the 2 clearing agents used for both embedding and mounting procedure?

Answer 13

Xylene/xylol
Toluene

Question 14

What is the clearing time for xylene?

Answer 14

30-60 minutes

Question 15

What is the clearing time for toluene?

Answer 15

1-2 hours

Question 16

What clearing agent is:
-a susbtitute for xylene or benzene
-slow penetration than xylene and benzene
-also used both for embedding and mounting process
-not carcinogenic

Answer 16

Toluene

Question 17

What clearing agent is:
-recommended for URGENT BIOPSIES
-carcinogenic
-causes damage to BM and Aplastic anemia
-most RAPID AND RECOMMENDED

Answer 17

Benzene

Question 18

This cclearing agent does not have the ability to harden the tissue even if it is soaked for 24 hours

Answer 18

Toluene

Question 19

What is the advantage and disadvantage of xylene?

Answer 19

Xylene is cheap but when stained for 2-3 hours, it hardens the tissue

Question 20

What is the disadavntage of Toluene?

Answer 20

Slow penetration and expensive

Question 21

If benzene is the most rapid clearing agent, how long is the clearing time?

Answer 21

15-60 minutes

Question 22

What clearing agent is:
-slower in action
-may cause hepatotoxicity
-does not make tissue transluscent but removes alcohol

Answer 22

Chloroform

Question 23

What is the clearing time for chloroform?

Answer 23

6-24 hours

Question 24

What clearing agent is:
-recommended for CNS TISSUE AND CYTOLOGY
-no tissue distortion even if left in oil
-VERY EXPENSIVE
-clearing agent for paraffin and celloidin sections

Answer 24

Cedarwood Oil

Question 25

What is the clearing time for cedarwood oil?

Answer 25

2-3 days

Question 26

What clearing agent is: -Not for routine purposes -recommended for EMBRYOS, INSECTS, and VERY DELICATE TISSUES

Answer 26

Aniline Oil

Question 27

What clearing agent is: -Similar to chloroform but cheaper -Highly toxic upon prolonged exposure

Answer 27

Carbon tetrachloride

Question 28

What clearing agent is: -Causes MINIMUM SHRINKAGE -Becomes adulterated (madali palitan) -EXPENSIVE

Answer 28

Clove oil

Question 29

What clearing agent is: -slow acting -for DOUBLE embedding techniques -Paraffin them embedding media

Answer 29

Methyl Benzoate and Methyl Salicylate

Question 30

What clearing agent is: -no dealcoholization but make the tissues clearer

Answer 30

Glycerine/gum syrup

Question 31

How many stations in clearing?

Answer 31

2-3 (2 standard)