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FNH 413 > Classmate MC Qs > Flashcards

Classmate MC Qs Flashcards

1
Q

Which antimicrobial below is least effective against Listeria monocytogenes?
* Bacteriophage
* Sodium lactate
* Nisin
* Salt

A

Salt

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2
Q

Which step during the processing of cooked ham is nisin applied?

A

Brining

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3
Q

What was the mortality and hospitalization rate (respectively) from Listeria monocytogenes according to a 2022 report from the US Food and Drug Administration?

A

20-30% mortality
94% hospitalization rate

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4
Q

In what order should a plant be cleaned and sanitised?

A) Rinsing, Washing, Sanitizing, Rinse/Air dry, Sweeping and flushing, Inspection, Validation

B) Inspection, Sweeping and flushing, Washing, Rinsing, Sanitising, Rinse/Air dry, Validation

C) Sweeping and flushing, Washing, Rinsing, Sanitizing, Rinse/Air dry, Inspection, Validation

D) Inspection, Sweeping and flushing, Rinsing, Washing, Sanitizing, Rinse/Air dry, Validation

A

B) Inspection, Sweeping and flushing, Washing, Rinsing, Sanitising, Rinse/Air dry, Validation

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5
Q

What are the 3 active ingredients in Oregano essential oil?

A

Linalool
Carvacrol
p-cymene

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6
Q

What important chemical compound is released during the fermentation of salami?

A

Lactic acid

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7
Q

Does nitrite decrease or increase antimicrobial activity of LAB?

A

Decreases

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8
Q

Why is Bacteriophage ListShield effective against Listeria monocytogenes ? [2]

A
  • It contains naturally occuring bacteriophages that selective and specifically kill Listeria monocytogenes
  • It contains a cocktail of 6 lytic bacteriophages that can overcome phage resistance
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9
Q

Why are sodium diacetate and sodium lactate effective against Listeria monocytogenes ? [3]

A
  • They reduce intracellular pH and reduce proton motive force
  • They change the transcription of genes that encode protein involved in membrane transport and energy metabolism
  • They lead to metabolic pathways that generate less energy
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10
Q

Which antimicrobial strategy is suggested as a cost-effective option for controlling L. monocytogenes in RTE meat products like pepperoni?

A

Thymol

This was from group 8’s presentation specifically.

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11
Q

Which of the following steps is not included in pepperoni production?

A) Grinding of raw materials
B) Heating the raw materials
C) Mixing the ingredients
D) Stuffing in ingredients into the casin

A

B) Heating the raw materials

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12
Q

What condition is incorrect regarding how the 3M™ MDS system works?

A. The 3M™ MDS contains a minimum of 4 different primers that aids in DNA amplification.

B. The 3M™ MDS uses fluorescence detection method in combination with the LAMP DNA amplification method.

C. A lysis step is involved before DNA amplification in the 3M™ MDS.

D. The 3M™ Molecular Detection Instrument conducts DNA amplification and detection.

A

B. The 3M™ MDS uses fluorescence detection method in combination with the LAMP DNA amplification method.

It uses bioluminescence

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13
Q

What 2 components in a Fraser broth contribute to a colour change in the medium?

A

Esculin
Ferric ammonium citrate

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14
Q

What type of foodborne pathogen detection is 3M molecular detection?

A

Loop-mediated isothermal amplification (LAMP)

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15
Q

What are the 4 target organisms of 3M Molecular Detection?

A
  • L. monocytogenes
  • Salmonella
  • E. coli
  • Campylobacter
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16
Q

List 3 advantages of NEAR over PCR.

A
  • More cost effective as thermocycler is not required
  • Faster amplification process
  • Lower energy requirement
17
Q

The diagnostic sensitivity of the ANSR reader is low at 16 hours of enrichment, mainly due to […].

A

The diagnostic sensitivity of the ANSR reader is low at 16 hours of enrichment, mainly due to slow/insufficient growth in enrichment culture by target organism.

18
Q

What is the purpose of an internal positive control?

A

Prevent false negative results

19
Q

What mechanism does the VIDAS UP system use?

A

ELFA

20
Q

Which of the rapid detection methods does VIDAS® UP use?

A

ELFA

21
Q

Why does VIDAS utilize recombinant phage protein-based technology?

A

It makes the assay more sensitive and specific than traditional immunoassays

22
Q

What is the first step of the overall workflow of the BAX System?

A

Enrich samples

23
Q

What is the primary purpose of fluorescent dyes in real-time PCR?

A

To label and quantify the amplified DNA

24
Q

Name 3 common symptoms of E. coli O104:H4 infections.

A
  • Vomiting
  • Abdominal cramps
  • Fever
25
Q

What is the nucleic acid amplification test used to identify norovirus genotypes?

A

Reverse transcription polymerase chain reaction (RT-PCR)

26
Q

Which provinces in Canada were involved in the oyster outbreak?

A

BC, AB, and ON

27
Q

In a romaine lettuce outbreak, why was there no recall of the romaine lettuce?

A

Short shelf-life

FNH 413 (7 decks)

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