Classmate MC Qs Flashcards
Which antimicrobial below is least effective against Listeria monocytogenes?
* Bacteriophage
* Sodium lactate
* Nisin
* Salt
Salt
Which step during the processing of cooked ham is nisin applied?
Brining
What was the mortality and hospitalization rate (respectively) from Listeria monocytogenes according to a 2022 report from the US Food and Drug Administration?
20-30% mortality
94% hospitalization rate
In what order should a plant be cleaned and sanitised?
A) Rinsing, Washing, Sanitizing, Rinse/Air dry, Sweeping and flushing, Inspection, Validation
B) Inspection, Sweeping and flushing, Washing, Rinsing, Sanitising, Rinse/Air dry, Validation
C) Sweeping and flushing, Washing, Rinsing, Sanitizing, Rinse/Air dry, Inspection, Validation
D) Inspection, Sweeping and flushing, Rinsing, Washing, Sanitizing, Rinse/Air dry, Validation
B) Inspection, Sweeping and flushing, Washing, Rinsing, Sanitising, Rinse/Air dry, Validation
What are the 3 active ingredients in Oregano essential oil?
Linalool
Carvacrol
p-cymene
What important chemical compound is released during the fermentation of salami?
Lactic acid
Does nitrite decrease or increase antimicrobial activity of LAB?
Decreases
Why is Bacteriophage ListShield effective against Listeria monocytogenes ? [2]
- It contains naturally occuring bacteriophages that selective and specifically kill Listeria monocytogenes
- It contains a cocktail of 6 lytic bacteriophages that can overcome phage resistance
Why are sodium diacetate and sodium lactate effective against Listeria monocytogenes ? [3]
- They reduce intracellular pH and reduce proton motive force
- They change the transcription of genes that encode protein involved in membrane transport and energy metabolism
- They lead to metabolic pathways that generate less energy
Which antimicrobial strategy is suggested as a cost-effective option for controlling L. monocytogenes in RTE meat products like pepperoni?
Thymol
This was from group 8’s presentation specifically.
Which of the following steps is not included in pepperoni production?
A) Grinding of raw materials
B) Heating the raw materials
C) Mixing the ingredients
D) Stuffing in ingredients into the casin
B) Heating the raw materials
What condition is incorrect regarding how the 3M™ MDS system works?
A. The 3M™ MDS contains a minimum of 4 different primers that aids in DNA amplification.
B. The 3M™ MDS uses fluorescence detection method in combination with the LAMP DNA amplification method.
C. A lysis step is involved before DNA amplification in the 3M™ MDS.
D. The 3M™ Molecular Detection Instrument conducts DNA amplification and detection.
B. The 3M™ MDS uses fluorescence detection method in combination with the LAMP DNA amplification method.
It uses bioluminescence
What 2 components in a Fraser broth contribute to a colour change in the medium?
Esculin
Ferric ammonium citrate
What type of foodborne pathogen detection is 3M molecular detection?
Loop-mediated isothermal amplification (LAMP)
What are the 4 target organisms of 3M Molecular Detection?
- L. monocytogenes
- Salmonella
- E. coli
- Campylobacter
List 3 advantages of NEAR over PCR.
- More cost effective as thermocycler is not required
- Faster amplification process
- Lower energy requirement
The diagnostic sensitivity of the ANSR reader is low at 16 hours of enrichment, mainly due to […].
The diagnostic sensitivity of the ANSR reader is low at 16 hours of enrichment, mainly due to slow/insufficient growth in enrichment culture by target organism.
What is the purpose of an internal positive control?
Prevent false negative results
What mechanism does the VIDAS UP system use?
ELFA
Which of the rapid detection methods does VIDAS® UP use?
ELFA
Why does VIDAS utilize recombinant phage protein-based technology?
It makes the assay more sensitive and specific than traditional immunoassays
What is the first step of the overall workflow of the BAX System?
Enrich samples
What is the primary purpose of fluorescent dyes in real-time PCR?
To label and quantify the amplified DNA
Name 3 common symptoms of E. coli O104:H4 infections.
- Vomiting
- Abdominal cramps
- Fever
What is the nucleic acid amplification test used to identify norovirus genotypes?
Reverse transcription polymerase chain reaction (RT-PCR)
Which provinces in Canada were involved in the oyster outbreak?
BC, AB, and ON
In a romaine lettuce outbreak, why was there no recall of the romaine lettuce?
Short shelf-life
