CLASSIFICATION OF CULTURE MEDIA

Question 1

consistency of medium that contains no solidfying agent (broth, infusion, milk)

Answer 1

Liquid

Question 2

consistency of medium that is
liquefiable with 2-3% agar
non-liquefiable with chopped meat (anaerobe) ;rice grain (fungi)

Answer 2

solid

Question 3

consistency of medium that contains 0.5-1% agar; Red algae

Answer 3

semi-solid

Question 4

with known exact composition

Answer 4

synthetic

Question 5

contains at least 1 non-chemically defined component (blood, serum, yeast extract)

Answer 5

non-synthetic/complex

Question 6

recommended for organisms that cannot grow in cell free media

Answer 6

tissue

Question 7

mccoy cell

Answer 7

from mouse cell line

Question 8

A549 cell

Answer 8

lung carcinoma

Question 9

hep-2 cell

Answer 9

from laryngeal carcinoma

Question 10

vero cell

Answer 10

african green monkey

Question 11

supports the growth of non-fastidious organism ex. nutrient agar or broth. trypticase soy agar or broth

Answer 11

simple/general purpose

Question 12

culture media that cotains nutrient supplements
example
blood agar plate: 5% defribinated sheep blood (horse/rabbit) chocolate agar plate

Answer 12

enriched culture media

Question 13

select the growth of particular organism and prevents other organism thru inhibitors
ex.
lowenstein jensen: mycobacterium tubercolosis
phenylethyl alcohol: gram positive microorganism
thayer martin, martin lewis, new york city: neisseria gonorrhea

Answer 13

selective culture media

Question 14

can enhance the growth of microorganism;
ex.
alkaline peptone water for vibrio
selenite and tetrathionate broth for salmonella and shigella

Answer 14

enrichment culture media

Question 15

allow certain types of organisms to grow and differentiate, and inhibit the growth of other organisms
ex.
mannitol salt agar: s. aureus and s. epidermidis/saprophyticus
thiosulfate citrate bile salt: vibrio spp.
salmonella-shigella agar; eosin methylene blue

Answer 15

selective-differential culture media

Question 16

provides distinct colonial appearance of microorganisms to know their identification
ex: TSI (triple sugar iron) contains 3 sugars (lactose, glucose, sucrose)

Answer 16

differential culture media

Question 17

blood agar plate contains

Answer 17

5% defribinated sheep blood (horse/rabbit) chocolate agar plate

Question 18

select the growth of particular organism and prevents other organism thru inhibitors

lowenstein jensen:
phenylethyl alcohol:
thayer martin, martin lewis, new york city:

Answer 18

mycobacterium tubercolosis
gram positive microorganism
neisseria gonorrhea

Question 19

alkaline peptone water enhances what microoganism

Answer 19

vibrio

Question 20

selenite and tetrathionate broth enhances what microorganism

Answer 20

salmonella and shigella

Question 21

provides distinct colonial appearance of microorganisms to know their identification

Answer 21

TSI (triple sugar iron)