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Micro > APPEARANCE OF ENTEROBACTERIACEAE ON SELECTED MEDIA > Flashcards

APPEARANCE OF ENTEROBACTERIACEAE ON SELECTED MEDIA Flashcards

1
Q

What does TSI stand for in microbiological media?

A

Triple Sugar Iron

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2
Q

What are the three sugars present in TSI media?

A

Glucose, lactose, and sucrose

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3
Q

True or False: Enterobacteriaceae can ferment lactose in MacConkey agar.

A

True

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4
Q

What color indicates lactose fermentation on MacConkey agar?

A

Pink

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5
Q

What type of bacteria does HEKTOEN Enteric agar primarily differentiate?

A

Enteric Gram-negative bacteria

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6
Q

What color do H2S-producing bacteria turn on HEKTOEN Enteric agar?

A

Black

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7
Q

Fill in the blank: XLD agar is selective for __________.

A

Enterobacteriaceae

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8
Q

What does the ‘X’ in XLD agar stand for?

A

Xylose

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9
Q

What color indicates xylose fermentation on XLD agar?

A

Yellow

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10
Q

True or False: TSI media can be used to detect hydrogen sulfide production.

A

True

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11
Q

What indicates a gas production in TSI media?

A

Cracks or lifting in the agar

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12
Q

What is the pH indicator used in TSI media?

A

Phenol red

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13
Q

What color does TSI turn when acid is produced?

A

Yellow

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14
Q

Which Enterobacteriaceae is characterized by producing a blue-green pigment on HEKTOEN Enteric agar?

A

Enterobacter aerogenes

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15
Q

What is the selective agent in MacConkey agar?

A

Bile salts and crystal violet

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16
Q

Fill in the blank: Non-lactose fermenters on MacConkey agar appear __________.

17
Q

What does a yellow slant and yellow butt in TSI indicate?

A

Fermentation of glucose and lactose/sucrose

18
Q

True or False: Salmonella produces a red colony on XLD agar.

19
Q

What is the appearance of Shigella on MacConkey agar?

A

Colorless colonies

20
Q

What does HEKTOEN Enteric agar differentiate between?

A

Lactose fermenters and non-fermenters

21
Q

When testing for Enterobacteriaceae, which media is used to identify hydrogen sulfide production?

A

TSI and HEKTOEN Enteric agar

22
Q

What does a black precipitate in TSI media indicate?

A

Hydrogen sulfide production

23
Q

What color do fermenters appear on XLD agar?

24
Q

What is the main carbohydrate source in XLD agar?

25
Q

True or False: All Enterobacteriaceae are lactose fermenters.

26
Q

How can you differentiate E. coli from other lactose fermenters on MacConkey agar?

A

E. coli produces a pink colony with a metallic sheen

27
Q

TSI: A/A, gas
MacConkey: flat, dry pink halo
Hektoen Enteric: yellow
XLD: Yellow

28
Q

TSI: K/A
MacConkey: Colorless
Hektoen Enteric: green
XLD: colorless

29
Q

TSI: K/A, gas, H2S
MacConkey: colorless
Hektoen Enteric: colorless
XLD: red, yellow or colorless w or w/o black centers

A

Edwardsiella

30
Q

TSI: A/A or K/A gas, w or w/o H2S
MacConkey: Colorless @ 24hr, may become pink @48Hrs
Hektoen Enteric: colorless
XLD: red, yellow or colorless w or w/o black centers

A

Citrobacter

31
Q

TSI: K/A, gas, H2S
MacConkey: Colorless
Hektoen Enteric: Green
XLD: Red with black center

A

Salmonella

32
Q

TSI: A/A, gas
MacConkey: Pink mucoid
Hektoen Enteric: yellow
XLD: yellow

A

Klebsiella

33
Q

TSI: A/A gas
MacConkey: pink may be mucoid
Hektoen Enteric: yellow
XLD: yellow

A

Enterobacter

34
Q

TSI: K/A
MacConkey: Colorless @ first, turning pink. S. marcescens may have red pigement at RT
Hektoen Enteric: colorless
XLD: yellow or colorless

35
Q

TSI: K/A (mirabilis) A/A (vulgaris) gas H2S
MacConkey: Colorless may swarm
Hektoen Enteric: colorless
XLD: yellow or colorless, w or wo black centers

36
Q

TSI: K/A gas
MacConkey: colorless
Hektoen Enteric: colorless
XLD: red or colorless

A

Morganella

37
Q

TSI: K/A
MacConkey: Colorless
Hektoen Enteric: Colorless
XLD: Yellow or Colorless

A

Providencia

38
Q

TSI: Yellow/ Orange
MacConkey: Colorless to peach
Hektoen Enteric: salmon
XLD: Yellow or colorless

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