Chromosomes, Cell Cycle, and Mitosis Flashcards
The continuity of life is based on the reproduction of what?
Cells, or cell division
What is a genome?
Genetic material of a cell
Describe a prokaryotic cell genome.
Single DNA molecule
Describe a eukaryotic cell genome.
Number of DNA molecules
Where are bacterial chromosomes located?
Nucleoid
What shape are bacterial chromosomes? How many?
Usually one circular chromosome
What is the role of proteins in chromosomes?
Hold the chromosome in place/ keep compacted
Where are eukaryotic chromosomes located?
Nucleus
What shape are eukaryotic chromosomes? How many?
Vary in number- a few to 100s in a linear shape
What are somatic cells?
(Nonreproductive cells) have two sets of chromosomes
What are gametes?
(Reproductive cells: sperm and egg) have half as many chromosomes as somatic cells= one set
How many pairs of chromosomes do humans have?
23
Each pair of homologous chromosomes includes _____ ______ _______ _______
One from each parent
What is a karyotype?
Ordered display of pairs of chromosomes from a cell
What are homologous chromosomes or homologs?
The two chromosomes in each pair
What are sex chromosomes? Where are they located?
X and Y, determine the sex of the individual. Located on the 23rd chromosome pair
What are autosomes?
remaining 22 pairs of chromosomes
What is a diploid cell? What is its symbol?
2 sets of chromosomes (2n)
What is the diploid number in humans?
46 (2n=46)
A gamete (sperm or egg) contains a single set of chromosomes and is thus a _____ _______
Haploid cell
An unfertilized egg (ovum) has a ___ chromosome and a sperm cell carries a ___ or ___ chromosome.
X
X or Y
A duplicated chromosome is known as what?
Sister chromatid
What is the centromere?
Narrowing in center of chromosomes
What are non-sister chromatids?
One chromosome from each homologous pair that are NOT identical
What is the first level of chromatin packing?
Nucleosome (10 nm in diameter)
What is the protein wrapped within the chromatin?
Histone
What is a chromatin?
DNA combined with a protein
What is the second level of chromatin packing? Describe the structure
30 nm fiber
More densely packed than 10 nm nucleosomes
What is the third level of chromtin packing?
Looped domain and scaffold
In what phase are most chromatin loosely packed in the nucleus?
Interphase
What is euchromatin?
Loosely packed chromatin (10-nm fiber)
What is heterochromatin?
Highly condensed regions of chromatin, centromeres and telomeres
If the chromatin is more condensed what does this mean for gene expression?
More difficult
In preperation for cell division, DNA is _______ and chromosomes _________.
Replicated
Condensed
What are cohesins?
Proteins that allow the chromosomes to stick together
How does bacteria preform cell division?
Binary fission
How does eukaryotic cells preform cell division?
Mitosis
What is the order of bacterial binary fission? (4)
- Chromosomes begin to replicate
- One copy of origin is now at each end of the cell (pulling toward edge)
- Replication finishes
- Two daughter cells result
Why do multicellular eukaryotes preform cell division? (3)
- Development from a fertilized egg
- Growth
- Repair
Most cell division reults in ___ ____ ____ with identical genetic information.
Two daughter cells
What is the result of meiosis? (2)
- Special type of division that can produce sperm and egg cells
- Yields nonidnetical daughter cells (n) that have half as many chromosomes as partent cells
What occurs in the Mitotic (M) phase
Cell division
What occurs in Interphase?
Cell growth and copying of chromosomes
What is the longest phase of cell division?
Interphase makes up 90% of the cell cycle
What is mitosis?
Division of genetic material in nucleus
What is cytokinesis?
Division of cytoplasm
What occurs in the G1 phase?
“First gap” metabolic growth, organelles growth, cell growth
What occurs in the S phase?
DNA replication
What occurs in the G2 phase?
“Second gap” last step of mitosis, checking for errors in DNA replication, organelles replicated
Define mitosis.
Transfer of genetic material
What are the five stages of mitosis?
- Prophase
- Prometaphase
- Metaphase
- Anaphase
- Telophase
What is the role of the nucleolus?
rRNA production
What is the centrosome?
Only in animal cells, microtubule-organizing center
Duplicated during G2
What occurs during prophase?
Chromosomes start to condense and centrosomes move to poles and microtubules extend
What is aster?
Radial array of short microtubules; extends from each centrosome
What is the mitotic spindle?
Stricture made of microtubules that controls the chromosome movement during mitosis
What makes up the mitotic spindle? (3)
Centrosomes, spindle microtubules, and asters
When does the nuclear envelope start to break down?
Prometaphase
What are kinetochores?
Protein complex associated with centromeres (attach to chromosomes)
What is the centromere?
Where daughter chromosomes are connected
What is the metaphase plate?
Chromosomes lined up at a plane midway between the spindle’s two poles
What are nonkinteochore microtubules?
Microtubules that do not attach to the chromosomes at the kinetochore.
What is separase?
Enzyme that cleves cohesins
What is the role of motor proteins?
Involves movement and the depolymerization of microtubules
What are the main two differences in plant and animal cell division?
- No centrioles (centrosomes)
2. Difference in cytokinesis
What structure is formed during cytokinesis in animal cells? Plant cells?
Cleavage furrow (animal) Cell plate (plant)