Chromosome Analysis: DNA fibre FISH

Question 1

T/F. Microscopic chromosome analysis at the highest resolution is achieved by techniques that release the elementary chromosome fibre from its associated proteins within the chromosome scaffold.

Answer 1

True.

Question 2

Greatly extended fibres of several megabases in length may be obtained by ____ of chromatin using ____.

Answer 2

decondensation; detergents and enzymes.

Question 3

In chromosome analysis method, DNA probes are hybridised by FISH to elementary fibres fixed onto microscope slides, allowing sequences less than 5kb apart to be separated readily, and distances down to 1 kb to be claimed.

Answer 3

DNA fibre FISH

Question 4

Fibre preparations have been particularly useful in determining the _____ and other types of _____, and in _____.

Answer 4

order and arrangement of centromeric heterochromatin; heterochromatin; high-resolution gene mapping