Chromatography Part A Obj 1

Question 1

How does chromatography determine the composition of mixtures despite using a non-specific
detector?

Answer 1

Chromatography separates mixtures into different components and measures the time it takes the components to pass through a detector

Question 2

Name and describe the two “phases” involved in chromatographic separation.

Answer 2

1) Stationary phase: holds back the components
2) Mobile phase: moves components through the stationary phase

Question 3

Where is Gas Chromatography typically used?

Answer 3

Oil refineries and hydrocarbon mixtures

Question 4

The term Gas Liquid chromatography specifies what about the measurement system design?

Answer 4

Gas is the mobile phase and liquid is the stationary phase

Question 5

What are 2 events that occur in the column during gas chromatography analysis?

Answer 5

1) Gas dissolves and absorbs into the stationary liquid phase
2) Mobile gas desorbs the components from the liquid stationary phase

Question 6

How do components behave differently in a chromatography system based on their solubility in the stationary phase?

Answer 6

The more soluble a component is the more time it spends in the stationary phase therefore the slower it moves through the column