Chromatography

Question 1

Stationary phase of TLC?

Answer 1

Thin layer of solid coated on an inert

Question 2

Mobile phase of TLC?

Answer 2

A liquid solvent which moves up vertically up the TLC plate

Question 3

Basis of separation of TLC?

Answer 3

The distance a component moves up the plate depends on how strongly it is adsorbed onto stationary phase
Weaker adsorption the further it will move

Question 4

Stationary phase of GC?

Answer 4

High boiling point liquid coated on the inside of a column

Question 5

Mobile phase of GC?

Answer 5

An inert gas

Question 6

What is retention time? GC

Answer 6

Time for a component to pass from the column inlet to the detector, measured from zero to the centre of the peak

Question 7

Basis of separation of GC?

Answer 7

The speed of a component moves through the column depends on how soluble it is in the stationary phase
More soluble the longer the retention time

Question 8

What is the peak area?

Answer 8

Area of each peak is proportional to the amount of that substance in the sample