Chromatography

Question 1

Define mobile phase:

Answer 1

The liquid or gas that moves (e.g. Solvent) and takes the mixture you are investigating with it

Question 2

Define stationary phase:

Answer 2

The object that the mobile phase moves through (e.g. Paper, beads)

Question 3

What are the lower blots more attracted to:

Answer 3

The stationary phase

Question 4

What are the higher blots more attracted to:

Answer 4

The mobile phase

Question 5

How many spots do pure chemicals have:

Answer 5

One

Question 6

How many spots do impure substances have:

Answer 6

More than one, but differ based on the solvent

Question 7

If a chromatogram has 4 spots, how many chemicals were in the mixture:

Answer 7

At least 4

Question 8

Define Rf value:

Answer 8

The retention factor. Can be compared to a database of values to find out which chemicals the mixture contains. The solvent used and temperature must be the same

Question 9

What is the formula for the Rf value:

Answer 9

Rf value =
distance moved by substance
———————-
Distance moved by solvent

Question 10

Does the unit matter in the Rf value formula:

Answer 10

No