Chromatography Flashcards
Utilizes a mobile liquid or gaseous phase that is adsorbed onto the surface of a stationary solid phase.
The equilibration between the mobile and
stationary phase accounts for the separation of different solutes.
Adsorption chromatography
Based on a thin film formed on the surface of a solid support by a liquid stationary.
Solute equilibrates between the mobile phase and the stationary liquid.
Partition chromatography
Use of resin is used to covalently attach anions or cations
Ion exchange chromatography
Lacks an attractive interaction between the stationary phase and solute
Molecular exclusion chromatography
Utilizes specific interaction between one kind of solute molecule and a second molecule that is immobilized on a stationary phase
Affinity chromatography
Thin layer chromatography.
Widely used.
Fast technique for qualitative analysis of a mixture of compounds.
Thin layer chromatography
High performance thin layer. Sophisticated and automated form of TLC.
High-Performance Thin Layer Chromatography
Utilizes vertical glass column filled with some form of solid support
Column chromatography
This is a fast, simple, widely used preparative separation technique, where the stationary bed is packed in a long, narrow glass lube.
Flash Column Chromatography
Versatile natural product isolation technique. Similar to flash chromatography.
High pressure (up to 4,000–5,000 psi) is applied to the system to move the mobile phase through the smaller particle sized (2–10
μm) stationary phase bed.
High-Performance Liquid Chromatography
Widely used chromatographic technique to analyze volatile compounds.
Gas chromatography
widely used, fast technique for the qualitative analysis of a mixture of compounds.
The stationary phase consists of a thin layer of adsorbent like silica gel, alumina, or cellulose on a flat carrier like a glass plate, a thick aluminium foil, or a plastic sheet. has certain advantages over PC.
Thin Layer Chromatography
