Chromatography 1.3 Flashcards
1
Q
What is chromatography used to do?
A
Separate chloroplast pigments according to their solubility and attractions
2
Q
How do you calculate Rf value?
A
Distance moved by pigment / distant moved by solvent
3
Q
Why is pencil used and not ink pen?
A
Does not interact/ travel up with the solvent
4
Q
Describe the method by which pigment is concentrated on the origin line?
A
Spot extract, allow to DRY and repeat
5
Q
Describe how you could confirm that your identification of one pigment using RF value was correct?
A
Spot each pigment on same paper and compare position with original sample
