Chromatography Flashcards
1
Q
What is chromatography used for?
A
- separating mixtures
- ie ink
2
Q
What is the mobile phase?
A
the solvent
3
Q
What is the stationary phase?
A
the paper
4
Q
How does chromatography work?
A
Mobile phase moving through stationary phase
5
Q
How do you know if a substance is the most soluble?
A
It moves the farthest up the paper
6
Q
How do you know if a substance is the least soluble?
A
It travels the least farthest uo the paper
7
Q
How are the substances separated (based on phases)?
A
Substances separated based on how attracted they are to each phase
8
Q
What does Rf stand for?
A
Retention factor
9
Q
Rf equation?
A
distance moved by substance/distance moved by solvent
10
Q
What is the maximum Rf?
A
1.0
11
Q
Give a use of Rf values
A
- can be used to identify unknown substances by comparing them to known samples
12
Q
What are you trying to find out in the RP?
A
- what substances make up and unknown substance
- by comparing unknown to known substances
