Cholesterol Flashcards
How many carbon atoms compose cholesterol?
27 carbon atoms
What is the structure of cholesterol?
27 carbon atoms, consists of cyclic rings with a hydrophobic tail steroid ring
Carbon and hydrogen,
C3 hydroxyl group
Why is cholesterol important?
Fluid dynamics within phospholipid bilayer, enables mechanical stability & fluidity
How does cholesterol maintain fluid stability within the phospholipid bilayer?
The cholesterol ring system interferes with motions of the fatty acid side chains
What is the first step of cholesterol synthesis?
Isopentenyl pyrophosphate- activated isoprene synthesis
What is the second stage of cholesterol synthesis?
Condensation of 6 molecules of isopentenyl pyrophosphate to form squalene (cytoplasmic reactions)
What is the fate of squalene?
Squalene undergoes demethylation and cyclisation to give cholesterol by monooxygenases
How is isopententyl pp formed (stage 1 &2)?
Two molecules of acetyl-CoA condense to form four carbon acetyoacyl-CoA
This is added to acetyl-CoA
Forms 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA)
What happens to HMG-CoA?
HMG-CoA reductase reduces HMG-CoA into Mevalonate
Which molecule provides the reducing power for the reduction of HMG-CoA into Mevalonate?
NADP+
Where does reduction HMG-CoA occur within a cell?
Within the cytoplasm
What is the fate of mevalonate?
Undergoes sequential phosphorylation at hydroxyl groups attached to c3 and c5 by kinase; decarboxylated into Isopenenyl-PP
What molecule is consumed for the phosphorylation of mevalonate?
ATP
What is the first step of squalene formation?
2 molecules of isopentenyl-PP condense to form dimethylallyl-pp
What happens to dimethylallyl-pp?
Addition of isopentenyl to form 15 carbon species