Chemiluminescence etc

Question 1

Luminometer - define and components?

Answer 1

Instrument used to measure chemiluminescence and electrochemiluminescence.
Components:
1. Sample cell housed in a light-tight chamber
2. Injection system to add reagents to sample cell
3. Detector, usually a photomultiplier tube

Question 2

Limitations of chemiluminescence etc

Answer 2

Light leaks
Light piping
High background linescence from reagents and reaction vessels
Impurities in water, reagents
Inefficient mixing of reagents upon injection into sample, resulting in loss of light capture
Pulse pile-up in PMTs causing underestimation of luminescence