Chapter Somtic Cell Nuclear Transfer Flashcards
What is nuclear transfer?
Nuclear transfer is a technique used to create a genetically identical copy of an animal. [1] In the image, it refers to the process that created Dolly the sheep.
What does nuclear transfer commonly involve?
Nuclear transfer commonly involves the transfer or placement of a donor nucleus into the cytoplasm of an enucleated MII oocyte.
In the nuclear transfer method, what is transferred into the cytoplasm of an enucleated MII oocyte?
In the nuclear transfer method, the donor nucleus is transferred into the cytoplasm of an enucleated MII oocyte.
How was the sheep Dolly generated?
The sheep Dolly was generated from an enucleated (nucleus was removed) egg into which the nucleus from a cultured somatic cell of a mature sheep was introduced.
What type of cell’s nucleus was introduced into the enucleated egg to create Dolly?
The nucleus from a cultured somatic cell of a mature sheep was introduced into the enucleated egg to create Dolly.
What steps are involved in the preparation of the egg/oocyte for research or medical procedures?
A target egg in the antral stage exhibiting the first polar body is selected. Follicles must be at least 18mm wide.
Human chorionic gonadotropin (hCG) is injected into the female donor to induce ovulation, providing a more comfortable way of obtaining the egg without invasive surgery.
Once the egg is extracted, it is placed in liquid human serum.
Fluorescent tags are bound to the oocyte’s DNA to ensure that the oocyte’s DNA/nucleus has been removed, which can be verified by exposing the egg to UV light.
