Chapter 9 - Cellular Respiration Flashcards
Step 2 of Citric Cycle
Citrate is converted to isocitrate by removing H2o and adding another
Step 1 of Citric Cycle
2 carbon acetyl group to Oxaloacetate produces citrate
Step 3 of Citric Cycle
Isocitrate is oxidized making NAD+ to NADH, losing a CO2
Step 4 of Citric Cycle
Alpha-ketoglutarate loses a CO2 and NAD+ becomes NADH and molecule attached to coenzyme A (Succinyl CoA)
Step 5 of Citric Cycle
CoA displaced by phosphate group transferred to GDP forming GTP (same with ADP and ATP), forms succinate
Step 6 of Citric Cycle
2 hydrogen transferred to FAD forming FADH2 and oxidizing succinate to Fumarate
Step 7 of Citric Cycle
H2O added to rearrange bonds to Malate
Step 8 of Citric Cycle
Substrate oxidized making NAD+ to NADH and regenerating oxaloacetate
How many pyruvate per glucose?
2
Energy Investment Phase Enzymes
- Hexokinase
- Phosphoglucoisomerase
- Phosphofructokinase
- Aldolase
- Isomerase
Energy Investment Phase Intermediates
- Glucose
- Glucose 6-Phosphate
- Fructose 6-Phosphate
- Fructose 1,6-biphosphate
- Glyceraldehyde 3-Phosphate
- Dihydroxyacetone Phosphate (DHAP)
Energy Payoff Stage Enzymes
- Triose Phosphate Dehydrogenase
- Phosphoglycerokinase
- Phosphoglyceromutase
- Enolase
- Pyruvate Kinase
Energy Payoff Stage Intermediates
- 1,3-Biphosphoglycerate
- 3-phosphoglycerate
- 2-phosphoglycerate
- Phosphoenolpyruvate
- Pyruvate
Where is ATP used in Glycolysis?
Energy Investment
Where is ATP produced in Glycolysis?
Energy Payoff
What is Fermentation?
Partial degradation of glucose without oxygen
NADH ATP production
3 ATP
FADH2 ATP production
2 ATP
Substrate level phosphorylation?
ATP is made by direct transfer of a phosphate group from an organic sub-strate to ADP by an enzyme (glycolysis and citric cycle)
Where is most of the ATP produced and how?
Oxidative phosphorylation: the ETC produces hydrogen ions at each major protein (I-IV) which makes a high concentration gradient in the inter-membrane system. These hydrogen go to the ATP synthase and rotate on a motor like a windmill and go to the lower gradient in the mitochondrial matrix. This powers another rotation in the synthase that makes the ADP and phosphate produce ATP
Where does Glycolysis take place?
Cytosol
How much ATP is produced in Glycolysis?
2 ATP
Where does pyruvate oxidation occur?
Mitochondria
How much ATP does pyruvate oxidation produce?
0