Chapter 9 Flashcards

1
Q

Friedrich Miester

A

found nucleic acid from pus

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2
Q

Griffith’s experiment

A

Diplococcus pneumoniae infect bacteria, coat type was cause which is a protein. Absence of it created diseas

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3
Q

Avery experiment

A

“transforming principle”. Using protease created disease. Only DNA was left so it is the genetic material

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4
Q

Hershey and Chase

A

saw viruses inject radioactive DNA into cells

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5
Q

Levine

A

Discovered three parts of the nucleotide, sugar, phosphate and the base

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6
Q

Sugar in DNA is called

A

deoxyribose

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7
Q

phosphate group

A

phosphate group

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8
Q

base

A

A-T

G-C

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9
Q

Erwin Chargraff observed

A

complementary base pairs

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10
Q

Maurice Wilkins and Rosalind Franklin

A

indicated DNA is a helix from crystallography

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11
Q

Watson and Crick

A

made the model, helixes are antiparallel

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12
Q

5-3

A

5-3 in opposite directions

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13
Q

A gene

A

segment of DNA, directs RNA to code for protein

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14
Q

Histones

A

wind up DNA to make chromosomes. Unwinds when stuff happens

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15
Q

Semiconservative organization

A

old strand with new strand

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16
Q

helicase

A

unwinds parental DNA strands

17
Q

binding proteins

A

stabilize separate DNA strands

18
Q

Primase

A

adds a “primer to the DNA strand”

19
Q

DNA polymerase

A

binds nucleotides to form new strand

20
Q

DNA ligase

A

seals up Okazaki fragments and other “nicks”

21
Q

purines have

A

two rings

22
Q

pyrimidines have

A

one ring

23
Q

crystallography is like a chandelier because

A

it bounces light off the molecule. We can guess what the structure is based off of it

24
Q

restriction enzymes

A

“molecular scissors”

25
Q

agarose gel electrophoresis

A

separates DNA based by size

26
Q

Gel acts as a sieve

A

for smaller fragments to travel further

27
Q

DNA fingerprinting

A

use radioactive probes

28
Q

Enzymes have recognition sequence

A

cuts DNA molecule at the point

29
Q

Making DNA Visible

A

dye

30
Q

Reliability of DNA evidence

A

not certain