Chapter 9 Flashcards
When does replication in bacteria begin
initiator protein binds to an origin of replication and unwinds a short stretch of DNA,
Replication process?
1) initiator protein binds to an origin of replication and unwinds a short stretch of DNA, to which DNA helicase attaches.
2) DNA helicase unwinds the DNA at the replication fork,
3) single-strand-binding proteins bind to the single nucleotide strands to prevent secondary structures,
4) and DNA gyrase (a topoisomerase) removes the strain ahead of the replication fork that is generated by unwinding.
Purpose of primase
primase synthesizes short primers consisting of RNA nucleotides, providing a 3’OH group to which DNA polymerase can add DNA nucleotides.
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