Chapter 8: Gene Expression Flashcards
What is a genome?
All genetic material contained in an organism or a cell, including DNA existing as chromosomes within the nucleus, and DNA in mitochondria and chloroplasts (if present)
What is a gene?
Particular sections or organisms that carry instructions in the form of a code
What is polypeptide?
A polymer of many amino acids linked by peptide bonds; forms a protein or part of a protein (refer to figure 8.2.6 pg 156 for the process of polypeptide formation)
What is a codon?
A series of three adjacent nucleotide bases in DNA and mRNA; each codon specifies a particular amino acid to be added when a polypeptide is assembled; start and stop codons also occur
What is a ribosome?
Organelle where polypeptide synthesis occurs in all cells; locks onto mRNA molecule and moves along it to translate its code and link amino acids; formed in the nucleolus
What is messenger RNA (mRNA)?
A ribonucleic acid molecule formed in the nucleus during gene transcription; has a nitrogen base sequence complementary to DNA template segment; travels to the cytoplasm where ribosomes attach
What is a template strand?
The DNA strand that serves as a pattern for making complementary polynucleotide
What is transcription?
The formation of an mRNA molecule against the template strand of DNA molecule in the nucleus by complementary nucleotide base pairing (figure 8.2.2 pg 153 for more detail)
What is a non-template strand?
The DNA strand complementary to the template strand; does not form the pattern for the synthesis of a complementary polynucleotide
What is pre-mRNA?
An unmodified ‘immature’ mRNA molecule that contains introns
What are introns?
A section of DNA or pre-mRNA that does not code for a polypeptide; is removes (spliced) from pre-mRNA to form a mature mRNA molecule
What is an exon?
A section of DNA, pre-mRNA, or mRNA that codes for a polypeptide
What is mature mRNA?
A modified pre-mRNA molecule with introns removed; ready to move out of the nucleus to the cytoplasm
What is a start codon?
The first codon of an mRNA transcript translated by a ribosome; signals the ribosome to start translating mRNA
What is translation?
The joining of amino acids in a specific order, according to information in mRNA ‘read’ by the ribosome, to form polypeptide
What is transfer RNA (tRNA)?
An RNA molecule that picks up a particular amino acid from the cytoplasm and then pairs with a specific mRNA codon to deliver the amino acid to the growing polypeptide chain