Chapter 8: Carbohydrate Metabolism Flashcards
What is glycolysis??
- process of converting glucose to two molecules of pyruvate capturing a small amount of energy
What is glycogenesis?
when glycogen ( a storage form) is formed when glucose levels are really high
What is glycogenolysis ?
when glycogen is degraded forming glucose for when glucose levels are very low.
What is gluconeogenesis?
when glucose is synthesized from noncarbohydrate precursors by reactions.
What is the pentose phosphate pathway?
enables cells to convert glucose-6-phosphate a derivative of glucose to ribose-5-phosphate and other types of monosaccharides
When glucose molecules are not required for immediate energy production it is stored as what??
-glycogen in the liver and muscles
Glycolysis is an ____ process??
anaerobic process which would have been needed in an oxygen poor environment
The small amount of energy captured in glycolysis is temporarily stored where?
- ATP and NADH
Anaerobic organisms??
- those that do not use oxygen to generate energy
Pyruvate can be converted into waste products such as what? (3)
ethanol, lactic acid and acetic acid
Aerobic respiration??
when oxygen is sued as a terminal electron acceptor….via completely oxidizing pyruvate to form CO2 and H2O in an elaborate stepwise mech.
Glycolysis consists of how many reactions and stages?
- 10 reactions
- 2 stages
Do a brief overview of what happens in each stage of glycolysis?
- Glucose —-> Glyceraldehyde-3-phosphate..consuming two ATP
- Glyceraldehyde-3-phosphate—-> 2 pyruvates
L> 4 ATP and 2 NADH
L> technically the net gain of ATP is actually 2
What is the net equation for glycolysis?
D-Glucose + 2 ADP + 2Pi + 2NAD+ —–> 2 pyruvate + 2 ATP + 2 NADH + 2H+ + 2H2O
What occurs in reaction one for glycolysis?
Glucose —> Glucose-6-phosphate
- 1 ATP in and 1 ADP via hexokinase and Mg 2+
What occurs in reaction two for glycolysis?
Glucose-6-phosphate fructose-6-phosphate
L> via phosphoglucoiosmerase
What occurs in reaction three for glycolysis?
Fructose-6-phosphate——> Fructose-1,6-bisphosphate
L> via PKF-1 ( phosphofructokinase-1)
L>One ATP in and one ADP out
What occurs in reaction four for glycolysis?
Fructose-1,6-bisphosphate Dihydroxyacetone phosphate (DHAP) + Glyceraldehyde-3-phosphate
L> via aldolase
What occurs in reaction five for glycolysis?
—> DHAP Glyceraldehyde-3-phosphate via triose phosphate isomerase
What occurs in reaction six for glycolysis?
Glyceraldehyde-3-phosphate Glycerate-1,3-bisphosphate
L> via glyceraldehyde-3-phosphate dehydrogenase
L> Pi+ NAD+ in and NADH + H+ out
What occurs in reaction seven for glycolysis?
Glycerate-1,3-bisphosphate glycerate-3-phosphate
L> via phosphoglycerate kinase
L> one ADP in and one ATP out
What occurs in reaction eight for glycolysis?
Glycerate-3-phosphate Glycerate-2-phosphate
L> via phosphoglycerate mutase
What occurs in reaction nine for glycolysis?
Glycerate-2-phosphate phosphoenolpyruvate (PEP)
L> enolase
(H2O out)
What occurs in reaction ten for glycolysis?
phosphoenolpyruvate ———-> Pyruvate
L>via pyruvate kinase
L> ADP in and ATP out
What are the products of glycolysis for one molecule of glucose?
2 ATP, 2 NADH, 2 Pyruvate
Under aerobic conditions what happens to pyruvate?
- it is converted into acetyl-CoA, which is the entry level for the citric acid cycle.
L> which is an amphibolic pathway which completely oxidizes 2 carbons to form CO2 and NADH and FADH2
The electron transport system??
a series of oxidation reduction reactions, transfers electrons from NADH and FADH2 to O2 to form water. The energy that is released during ETC is coupled to a mechanism that synthesizes ATP
Under anaerobic conditions what happens to pyruvate?
- further oxidation of pyruvate is impeded.
L> compensated via converting it to a more reduced organic compound and regenerating the NAD + required for glycolysis to continue via fermentation
ex: muscle cells produce NAD + via pyruvate Lactate
In yeast what happens to pyruvate?
- decarboxylated to form acetaldehyde which is then reduced by NADH to form ethanol!
What three reactions in glycolysis have negative delta G values?
1, 3 and 10!
( catalyzed by hexokinase, PFK-1 and pyruvate kinase)
L> all of which are irreversible
What are the delta G values for the remaining reactions (2,4-9) in glycolysis?
- they are so close to zero that they operate near equilibrium.
L> therefore they are easily reversible; small changes in substrate or product concentrations can alter the direction of each reaction.
During gluconeogenesis which reactions does it bypass?
- it does not involve reactions 1, 3 and 10
L> therefore it does not involve those enzymes either…but it utilizes the rest of the glycolysis enzymes.
Glycolysis is regulated by what? (3)
- allosteric regulation of three enzymes : hexokinase, PFK-1 and pyruvate kinase.
L> the reactions catalyzed by these are irreversible and can be switched on and off by allosteric effectors
Glycolysis regulation:
What is hexokinase inhibited by?
- excess g-6-p
Glycolysis regulation:
- PFK-1 and pyruvate kinase are activated by what??
High AMP levels
indicator for low energy production
Glycolysis regulation:
What inhibits both PFK-1 and pyruvate kinase?
- high levels of ATP
Glycolysis regulation:
When ATP is in a high concentration citrate and acetyl-CoA accumulate and inhibit what?
- PFK-1 and pyruvate kinase
Glycolysis regulation:
Fructose-2,6-bisphosphate produced via hormone induced covalent modification of PFK-2 is an indicator of high levels of available glucose and allosterically activates what?
PFK-1
Glycolysis regulation:
Accumulation of fructose-1,6-bisphosphate activates what?
pyruvate kinase
Glycolysis is also regulated by what two hormones?
Glucagon and insulin