Chapter 8

Question 1

DTH recall antigens

Answer 1

Candida, tetanus, mumps

Standardized DTH allergens=Tuberculin, Candida

Question 2

Negative DTH test reasons

Answer 2

Anergy (cellular immunodeficiency, cancer, sarcoid, immunosuppressive therapy, severe atopic dermatitis, recent/recurrent infection), immediately after MMR vaccine, lack of exposure to antigen, weak antigen

Question 3

Excessive DTH reaction seen in

Answer 3

Mutations of IL-12, IL-12R, IFN gamma, IFN gamma receptor

Question 4

Washout periods for nasal provocation

Answer 4

Nasal antihistamines -3 days
Oral antihistamines - 3-10 days 
Nasal cromones -3 days
Nasal steroids - 7 days
Oral steroids - 7 days 
Leukotriene receptor antagonist - 7 days 
Alpha adrenergic agonist - 1 day

Question 5

Unilateral nasal provocation challenge increases what in the contralateral side

Answer 5

PGD2 and histamine (reflex activation of mast cells)

Secretion increase bilaterally, nasal airway resistance increases only on challenged side

Question 6

Nasal provocation with methacholine

Answer 6

Ipsilateral response, does not induce nasal reflexes

Question 7

Nasal provocation to histamine

Answer 7

Does not induce a late inflammatory response

Question 8

Western blot used for

Answer 8

Lyme dz, hhv6, hhv8, HIV confirmatory test, HTLV, prion disease, RSV, SARS, VZV, hep B confirmatory test,

Question 9

SPEP fractions

Answer 9

Albumin
A1- alpha 1 antitrypsin, thyroid binding globulin, trans Orrin, HDL
A2-ceruloplasmin, alpha 2 macroglobulin, haptoobulin
B1- transferrin
B2- beta lipoproteins
Gamma- immunoglobulins