Chapter 8

Question 1

four main types of transport

Answer 1

diffusion, thermal convection, flow, coupled transport

Question 2

HPLC: affinity based chromatography

Answer 2

Used to determine the amount of a certain separated species present in the original sample by first integrating area under the peak for that species.

Question 3

Colorimetric assays

Answer 3

There’s usually a linear correlation between the degree of color change and the amount of enzyme of interest

Question 4

Fluorescent assays

Answer 4

Advantages: fluorescent phenomena are detectable at one to three orders of magnitude lower concentration than that observable with colorimetric techniques.

Question 5

Enzyme-linked Immunosorbent assay (ELISA)

Answer 5

Used for very specific identification of a protein

1. The sample (containing possible protein of interest) is added
2. A second antibody that is conjugated to an enzyme is added. The secondary antibody will bind to another specific site on the protein of interest.
3. The substrate for the enzyme is added and color change is quantified on a plate reader/spectrophotometer. (color change is proportional to amount of protein present)

Question 6

Western Blotting

Answer 6

Separates proteins based on molecular weight. Larger proteins experience more drag so migrate more slowly.