Chapter 8 Flashcards
What aspect of tumor suppressors are we focusing on in chapter 8?
Tumor suppressors ability to promote differentiation.
What aspect of the processes that contribute to net cell number are we focusing on for this chapter?
Cells that become differentiated often enter G0 and do not undergo mitosis.
What does the word ontogeny mean?
It is the creation of a being - development of an individual.
Where do embryonic stem cells come from?
They are derived from the inner cell mass.
What do the hematopoietic stem cells give rise to?
They give rise to all the different blood cell types.
What does the term totipotent mean?
Totipotent is a type of stem cell that can become any single cell within the growing organism.
What does the term pluripotent mean?
This also refers to a stem cell’s ability to differentiate into any cell in the body with the exception of the extraembryonic cells that make up the placenta and the amniotic sac.
What is a progenitor cell?
Progenitor is another term that refers to the variety of cells that a stem cell can differentiate into. Here, it refers to a much more narrow ability of differentiation than the previously mentioned cells.
What is a precursor cell?
A precursor cell is the final term that refers to a stem cell’s ability to differentiate into other cell types. This is the most specific type in which these cells have a relatively certain fate.
Define differentiation.
Differentiation is the functional specialization of a cell as a result of the expression of a specific set of genes.
What does the term enucleation mean?
It refers to the characteristic of RBC’s not having a nucleus.
They also do not have a mitochondria!
All of the cells in the body contain the full complement of the human genome with two exceptions.
What are the exceptions?
How is this possible?
The exceptions are RBCs and germline cells.
This is made possible by different expressions among these cells.
How are stem cells important in adult life?
Give one example.
They are important for regeneration of tissues during life.
Example is hematopoietic stem cells that are continuously active to replace cells as they mature and die off.
HSCs show some differentiation plasticity.
What does this mean?
They can differentiate into cells that are not typical to their lineage under certain circumstances.
Even mammary cells can be reprogrammed to direct the development of a new individual - think dolly the cloned sheep.
What importance does this fact play?
The pattern of gene expression of a differentiated cell is not permanently fixed.
What are the two defining features of stem cells?
- They have the ability to self-renew
- the daughter cell maintains the characteristics of a stem cell
- They have the ability to give rise to committed progenitors of differentiated cell types - cells that typically withdraw from the cell cycle.
What are cancer stem cells (CSCs) ?
They are the cells within a tumor that have the ability to self-renew and to give rise to phenotypically diverse cancer cells.
Are cancer stem cells particularly common?
No, only a small percentage are cancer stem cells - the rest are differentiated.
What would happen if cancer stem cells were transplanted into a host animal?
They have the ability to initiate new tumors when transplanted - think about that one tumor that guy discovered putting cancer cells into chickens.
How can markers of stem cells be useful?
They are things that are expressed on the surface of the cell and are characteristic of the stem cell that is normally present in the tissue.
For normal HSCs, the markers CD34 and CD38 should be?
+ for CD 34
- for CD 38
The proportion of CSCs present often correlates with the prognosis of the cancer.
What does this mean?
This means that when there are fewer cancer stem cells, there is a better prognosis and when there are more cancer stem cells there is a worse prognosis.
What is the cancer stem cell model?
The model proposes that there are subpopulations of cells within the tumor that have stem cell properties that can initiate and maintain the cancer phenotype.
How was the cancer stem cell model demonstrated?
There have been experiments that demonstrate small populations within tumors that drive tumorigenesis.
How are cancer stem cells relevant to carcinogenesis?
- Their ability to self-renew provides increased opportunities for carcinogenic changes.
- If the system that normally regulates self-renewal gets disrupted, these cells can grow uncontrollably and cause cancer.
What normally happens to differentiated cells?
They will eventually die within days or months - they exit the cell cycle and eventually undergo apoptosis.