Chapter 7: Analysis Flashcards
What are the SECTIONS of the medical laboratory?
- Clinical Chemistry
- Hematology
- Immunology and Serology
- Blood Banking
- Histopathology
- Clinical Microscopy
- Molecular Diagnostics
It refers to many analytic determinations made in clinical laboratories that are based on the measurement of radiant energy that is absorbed or transmitted.
The devices used to measure absorbed or transmitted light energy are photometers and spectrometers.
Analysis: Principles of Instrumentation
What are the basic components of spectrophotometers?
- Radiant energy source
- Wavelength selector
- Cuvette holder
- Photodetector
- Signal processor
- Readout devices
The absorbance and percent transmittance are inversely related as given in the following equation.
A = 2 - log% T
True or False
(a) Monochromatic radiation absorbance is directly proportional to the path length.
(b) Through the medium and concentration.
(c) The absorbing species
True
What is the full equation of Beer-Lambert Law?
A = - logT = logP o/P = abc
Both visible and ultraviolet light are widely used in analytic methods.
Electromagnetic Radiation
It is used to measure analytes by measuring the quantity of light reflected by a liquid sample that has been dispensed onto grainy or fibrous solid support.
Reflectometer
It is the principle of automated urine analyzers in the laboratory.
Reflectance photometry
It is used to detect light that is scattered at various angles.
It is also used in detecting antigen-antibody complexes.
Nephelometer
It measures a reduction in light transmission due to particle formation.
Turbidimetry
