Chapter 7 Flashcards
How do you calculate total magnification
Ocular power x Objective power
(10x ocular lens, 4x objective lens) 10 x 4 = 40
How to calculate current FOV
(Original Magnification ÷ New magnification) x Original FOV
how is FOV determined
using a ruler or grid
how to calculate object size
( dFOV in mm ÷ number of time cell spans FOV )
convert metres to centimetres
÷ 100
convert centimetres to millimetres
÷ 10
convert millimetres to micrometres
x 1000
convert micrometres to nanometres
x 1000
advantages and disadvantages of the light microscope
+ Cheap, Small, Coloured images
- Less resolution and magnification
advantages and disadvantages of the electron microscope
+ Better quality, increase magnification
- Expensive, Black and white images, Large
what can the light microscope view
hair, Red Blood Cells, Bacteria, Some viruses
what can the electron microscope view
everything a light microscope can plus DNA molecules, Glucose molecules and atoms
how does a light microscope work
Light is transmitted through two lenses
how does an electron microscope work
Uses electron beam instead of light
define cytoplasm
Thick fluid within the cell membrane and all the structures suspended in it