Chapter 4: Recombination Frequency

Question 1

In an RFLP, the presence or absence of a restriction site produces what characteristics?

Answer 1

DNA fragments of varying lengths, reflecting sequence variation

Question 2

In VNTRs variation between two restriction sites in fragment lengths is due to what occurrence?

Answer 2

differences in number of tandem repeats located between two restriction sites

Question 3

In STR, how is variation in fragment length produced?

Answer 3

by differences in the number of micro-satellite repeats found between two PCR primer sites

Question 4

What are SNPs?

Answer 4

single differences in nucleotide sequences

Question 5

What does 1cM equate to?

Answer 5

centimorgan = 1% recombination frequency

1cM = 1 million base pairs

Question 6

When gene marker and gene remain on the same chromosome what are they referred to as?

Answer 6

linked

Question 7

What is the main rule for determining LOD scores?

Answer 7

LOD > 3.00 shows statistical evidence of linkage (more than 2 above 3.00 then use the score that is the highest)

LOD < -2.00 shows stastistical evidence of nonlinkage

LOD score between -2.00 and 3.00 is interdeterminate