Chapter 4 Flashcards
Microscope that can examine cells in living state
Dark-field
Microscope not useful
in the study of living microorganism
Transmission Electron
Refers to the ability of the lenses to distinguish fine two points as distinct and separate
Resolution
Achieved when light rays from an illuminator are passed through a condenser
Magnification
Refers to the light bending ability of the medium
Retractive index
Fine detail of objects such as viruses or bacterial flagella can be seen by thus technique
Negative staining
Used to make observations of intercellular structures, the material for examination must be extremely thin
Ultrathin sectioning
Developed to prepare sections of the specimen without resorting to the chemical treatment of the fixation process, which can produce artifacts
Freeze-Etching
special techniques developed making it possible to locate chemical constituents of the cell
Localization of the Cell Constituents
Limitations of an electron microscope
drying process may alter some morphological characteristics
Cytochemical method in which the location of a particular chemical constituent in a specimen is determined by observing the site at which radioactive material becomes positioned
Auto radiography
Soecific microscope that reveals bumps and depressions of the atoms on the surface
Scanning microscope
the ability of the lenses to distinguish two points as distinct and separate
resolution
a compound microscope that uses a computer to process the image to produce two or three dimensional images of the cells
scanning electron microscope
E. coli was named after?
Theodor Escherich