CHAPTER 3: STAINS AND STAINING METHODS Flashcards
- – coloring agent used for general purposes
Dye
dye used for biological purposes (specific
Stain
derived from coal tar
rendered synthetically to become a stain
aniline dyes
Colorless organic compound that can bind to the nitro
group of chromophores
Benzene
Chemical groups with conjugated double bonds,
imparts color to the colorless benzene.
Chromophore
Groups that intensify the color of chromophore by
conveying the property of ionization to the chromogen
and enabling it to form salts and binding to the
biological substance
Auxochrome
as “an organic compound
containing both chromophores and auxochrome link to the
benzene.”
stain
Natural stain example
▪ Example: hematoxylin, carmine
safranin stin example
▪ safranin, methylene blue, crystal violet, etc.
Direct stain example
▪ Ex. Aniline dyes