Chapter 3 MICRO

Question 1

what are the five I’s

Answer 1

inoculation, incubation, isolation, inspection, and identification

Question 2

describe inoculation

Answer 2

introduce sample of microorganism onto medium

try to create sterile inoculation

Question 3

describe incubation

Answer 3

medium is placed in temperature controlled incubator
can also control atmospheric gases
microbe multiplies

Question 4

what three ways can one classify a medium

Answer 4

physical state
chemical composition
functional type

Question 5

a defined media is

Answer 5

a media whose exact chemical compositions are known

Question 6

what a complex media

Answer 6

dont know the exact chemical composition

Question 7

what is an enriched medium

Answer 7

contains complex organic substances such as blood, serum, hemoglobin or special growth factors

Question 8

bacteria that require growth factors and complex nutrients are termed

Answer 8

fastidious

Question 9

what is a selective medium

Answer 9

contains one or more agents that inhibit the growth of certain microbe or microbes but not others
dyes, antimicrobial drugs

Question 10

what is a differential media

Answer 10

allow multiple types of microorganisms to grow but are designed to display visible differences among their colonies
colony size or color, media color changes, gas bubble or precipitates
chemicals, dyes, blood agar

Question 11

what is a reducing medium

Answer 11

contains a substance that absorbs oxygen or slows the penetration oxygen in a medium
anaerobic bacteria

Question 12

Trasport media is

Answer 12

used to maintain and preserve specimens that have to be held for a period of time before clinical analysis or to sustain delicate species that die rapidly if not held under stable conditions

Question 13

Assay media is used for

Answer 13

testing the effectiveness of antimicrobial drugs

Question 14

Describe isolation

Answer 14

used to grow a microbe and its colony

take small number of cells and spread over media hoping to isolate specific microbe

Question 15

what is a pure culture

Answer 15

a container of medium that grows only single known species or type of microorganism

Question 16

what is a mixed culture

Answer 16

container that holds two or more identified, easily differentiated species of microorganisms

Question 17

describe inspection

Answer 17

view specimen microscopically to observe characteristics

maybe have to stain slide

Question 18

describe identification

Answer 18

use relevant data to compare microbe to

biochemical tests

Question 19

label how the lens on microscopes go from light source to eyes

Answer 19

condenser lens, objective lens and ocular lens

Question 20

what is resolultion

Answer 20

distinguish or separate two adjacent objects or points from one another

Question 21

differential stains use

Answer 21

a primary dye and counterstain

Question 22

what are the four types of visible light microscopy

Answer 22

bright field, dark field, phase contrast and interference

Question 23

transmission electron microscope

Answer 23

transmit electrons through specimen

Question 24

scanning electron microscope

Answer 24

gives a 3D image of specimen, metal coated specimen