Chapter 3 - Cells And Movement In And Out Of Them Flashcards
Define “resolution”?
Minimum distance apart that two objects can be in order for them to appear as seperate items
Define “magnification”?
How many times bigger the image is when compared to the object
What is cell fractionation?
Where cells are broken up and their organelles are seperated
Why must a solution be cold?
To reduce enzyme activity
Why must a solution be isotonic?
To prevent bursting or shrinking as a result of osmosis
Why must a solution be buffered?
To maintain constant pH
What is homogenation?
Cells that are broken up by a homogeniser (blender) which releases organelles
Explain the following things about a light microscope;
- uses a beam of…
- beam is focused by…
- image forms on…
- resolution of…
- light
- lenses
- human eye
- 0.2 micrometers
Explain the following things about an electron microscope;
- uses a beam of…
- beam focused by…
- image forms on…
- resolution of…
- electrons
- magnets
- fluorescent screen
- 0.1 nanometers
Limitations of an electron microscope?
Needs a very thin specimen
limitations of a light microscope?
The specimen surface is important
What is the difference between prokaryotic and eukaryotic cells?
Eukaryotic; Prokaryotic;
Distinct nucleus No nucleus
Membrane bound organelles Membrane bound organelles
What is the function of epithelial cells?
To absorb and secrete
What is the function of a nucleus?
It acts a control centre of the cell through production of MRNA and hence aids in protein synthesis
Where would you find the nuclear envelope and what is it’s function?
Surrounding the nucleus. It is a double membrane. The outer membrane is continuous with ER and has a ribosomal surface.
Where would you find nuclear pores and what is it’s function?
On the nucleus surface and they allow passage of large molecules.
What is nucleoplasm?
Granular jelly like material found in the nucleus
What is a chromatin?
DNA found within the nucleoplasm.
What is a nucleolus?
Small sphere within the nucleoplasm, manufactures ribosomal RNA
What 3 things does a mitochondrion consist of?
- double membrane
- cristae
- matrix
What does a double membrane of a mitochondrion do?
Surrounds organelle and outer layer controls movement of materials. Inner membrane us is folded to form cristae
What does cristae do?
Provide large surface area for the attachment of enzymes involved in respiration
What does the matrix do?
Semi rigid material containing protein, lipids, and traces of DNA to control production of their own proteins.