Chapter 3 - Cell Structure Flashcards
what is an object
the material placed under the microscope
what is an image
the appearance of the material viewed under the microscope
why is the resolution of an electron microscope greater than that of an optical microscope
electrons have a very small wavelength compared to light
equation for magnification
magnification = size of image/size of real object
what is magnification
how much bigger the image is than the specimen you’re looking at
what is resolution
how well a microscope can distinguish between two points that are close together
what do optical microscopes use to form an image
light
what do electron microscopes use to form an image
electrons
maximum resolution of a light microscope
0.2 micrometres
maximum resolution of light microscopes
1500 x
maximum resolution of electron microscopes
0.0002 micrometres
maximum magnification of electron microscopes
1,500,000 x
what are the two types of electron microscopes
transmission electron microscopes TEM
scanning electron microscopes SEM
hoe do transmission electron microscopes work
a beam of electrons is focussed onto a specimen using electromagnets
the electrons are transmitted through the specimen
denser parts of the specimen absorb more electrons so they appear darker
what are the main limitations of TEM 3
they can only be used on thin specimens
the whole system must be observed in vacuum- living specimens can’t be viewed
a complex preparation process mans artefacts are common
what are artefacts
things that you can see on the microscope but aren’t part of your specimen
how many micrometers are in a millimetre
1000
how many nanometers in a micrometre
1000
how do scanning electron microscopes work
a beam of electrons is scanned onto the surface of a specimen
this knocks electrons off of the specimen which are gathered in a cathode ray tube to form an image
advantages of TEM
high resolution images mean you can see internal structures like chloroplasts
advantages of SEM
they can be used on thick and living specimens
disadvantages of SEM
the resolution is lower than TEM
what is an eyepiece graticule
a transparent rule with number but no units
how to calibrate a microscope
1) divide total length of stage micrometer by how many intervals to find one micrometer unit
2) find a point where the two lines are equal
3) work out how many micrometers units equal how many eyepiece units
4) convert the stage micrometre units to micrometers
5) divide stage units by eyepiece units to find the value of one eyepiece unit
6) this can be used in calculations
what is cell fractionation
the process where cells are broken up and different organelles they contain are separated out
during homogenisation why is the solution
a) cold
b) same water potential as the tissue
c) buffered
a) to reduce enzyme activity that could break down organelles
b) to prevent organelles bursting or shrivelling due to osmosis
c) any change in pH could affect the organelles or enzymes
what happens during homogenisation
cells are broken up in a blender to release organelles
what happens during filtration
the homogenate is filtered to remove any large sections of cell and debris
what happens during ultracentrifugation
the filtrate is poured into a test tube place in the centrifuge and spun slowly
the heaviest organelles sink to the bottom forming the pellet whilst the rest of the organelles stay suspended in a fluid called the supernatant
the supernatant is drained off, poured into another test tube and spun at an even higher speed
the next heaviest organelles form the pellet and are remove
description of the nucleus
nuclear envelope surrounding chromatin and nucleoplatism
function of the nucleus
stores genetic info DNA
decription of mitochondria
double membrane. inner highly folded-cristae
function of mitochondria
makes energy ATP rich molecules during aerobic respiration
description of cell surface membrane
free in cytoplasm attached to RER smaller in prokaryotic cells
function of cell surface membrane
makes proteins
rough size of mitochondria
between 2 and 10 um
what is the chloroplast envelope
a double membrane surrounding the organelle
what are grana
stacks of thylakoid membranes inside chloroplasts
what is stroma
a matrix where the sugar are synthesised during photosynthesis
function of chloroplasts
site of photosynthesis
how are chloroplasts adapted for photosynthesis
the grana provide a large surface area for the first stage of photosynthesis
chloroplasts contain ribosomes and DNA so they can quickly make proteins required for photosynthesis
what are the two types of endoplasmic reticulum
smooth endoplasmic reticulum SER
rough endoplasmic reticulum RER
what is the RER
a system of membranes covered in ribosomes
network of interlined sheets with ribosomes