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BRS Micro/Immuno > Chapter 3 Bacterial Genera > Flashcards

Chapter 3 Bacterial Genera Flashcards

1
Q

What is the first stain of Gram-staining?

A

crystal violet

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2
Q

What is the second stain of Gram-staining?

A

iodine

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3
Q

What is the third stain of Gram-staining?

A

alcohol

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4
Q

What is the fourth stain of Gram-staining?

A

safranin

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5
Q

What is the pneumonic for bacteria that do not grain stain?

A

CRUM LACE

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6
Q

Dark field microscopy is useful for detection of what bacteria?

A

spirochetes

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7
Q

What two bacteria species grow on chocolate agar?

A

Neisseria and Haemophilus

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8
Q

What bacteria grows on thayer martin?

A

Neisseria

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9
Q

TCBS media is used to grow what species of bacteria?

A

Vibrio

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10
Q

What two media are used to grow Bordatella pertussis?

A

Regan-Lowe and Bordet-Gengou

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11
Q

What media is used to grow Legionella?

A

BCYE

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12
Q

The Nagler test is used for what bacteria? What enzyme is being tested for?

A

Clostridium perfringens

lecithinase

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13
Q

Catalse test is used to differentiate what two bacteria?

A

Staph (+) and Strep (-)

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14
Q

Do gram-negative or gram-positive bacteria produce oxidase? What group of gram-negative bacteria do no produce oxidase?

A

Negative

Entero

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15
Q

Nitrites in the urine are indicative of what bacteria? What enzyme accomplishes this reaction?

A

enterobactericiae

Nitrate reductase

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16
Q

Chocolate agar is used to grow which two bacteria?

A

Neisseria and H. influenzae

17
Q

What UTI causing organism does not produce nitrate reductase?

A

S. saprophyticus

18
Q

What genus of bacteria is grown at 42C under microaerophilic conditions?

A

Camplyobacter

19
Q

What media can produce an oxygen gradient?

A

thioglycollate

20
Q

Is teichoic acid found in gram positive or gram negative bacteria?

A

gram positive

21
Q

Are streptococci catalase negative or positive?

A

negative

22
Q

Is S. epidermidis coagulase positive or negative?

A

negative

23
Q

Is S. saprophytiucus coagulase positive or negative?

A

negative

24
Q

Is S. pneumoniae bile-soluble or bile-insoluble?

A

bile soluble

25
Q

What is the most important bug in GAS?

A

Strep pyogenes

26
Q

What is the most important bug in GBS?

A

Strep. agalactiae

BRS Micro/Immuno (4 decks)

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