Chapter 3 Flashcards

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1
Q

What is a prokaryotic cell?

A

A cell which doesn’t contain membrane bound organelles

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2
Q

Example of prokaryotic cells

A

Bacterial cell

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3
Q

What is a eukaryotic cell?

A

Cells which contain membrane bound organelles

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4
Q

Example or eukaryotic cell

A

Animal cell

Plant cell

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5
Q

What are the differences of prokaryotic and eukaryotic cells?

A

Prokaryotic: no nucleus/DNA is in circular strands called plasmids/ ribosomes are smaller/capsule/no chloroplasts/cell wall made of murein
Eukaryotic:clear nucleus/DNA is histones/DNA is linear/chloroplasts/ribosomes larger/no capsule/cell wall made of cellulose

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6
Q

In a prokaryotic cell what does the capsule do?

A

Layer of polysaccharide that protects the cell

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7
Q

In a prokaryotic cell what does the cell wall do?

A

Maintains cell shape and rigidity

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8
Q

In a prokaryotic cell what does the plasma membrane do?

A

Lots of proteins and is responsible for the transport of proteins

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9
Q

In a prokaryotic cell what does the ribosomes do?

A

Gives a grainy appearance-it translates the genetic message in meg gage RNA into the production of proteins

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10
Q

In a prokaryotic cell what does the pill do?

A

Hollow hair like structures made of proteins which allow the bacteria to attach to other cells

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11
Q

In a prokaryotic cell what does the nucleotide do?

A

This is the area which the DNA is confined to because a prokaryotic cell has no nucleus

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12
Q

In a prokaryotic cell what does the flagella do?

A

Long appendages which rotate by means of a motor which is under the cytoplasmic membrane

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13
Q

How do cells divide in prokaryotic cells?

A

Binary fission

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14
Q

What is the process of Binary Fission

A

1) DNA replicates and attaches to cell membrane
2) plasmid replicates
3) cell membrane grows inward, pinching the cytoplasm
4) new cell wall forms between the 2 DNA molecules
5) 2 new daughter cells

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15
Q

What is in the ultra stutter of an animal cell?

A
Mitochondria
Cell membrane
Ribosomes 
Rough endoplasmic reticulum 
Smooth endoplasmic reticulum 
Lysosomes
Golgi body
Microtubules
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16
Q

What does the mitochondria do?

A

Responsible for aerobic respiration due to large surface area and folds

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17
Q

What does the cell membrane do?

A

Let’s substances in and out of the cell

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18
Q

What does the ribosomes do?

A

Found in the cytoplasm, they synthesis proteins for the cytoskeleton and general functions

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19
Q

What does the microtubules do?

A

Move cytoplasmic components

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20
Q

What does the RER do?

A

Ribosomes are attached to the cytosolic side of the membrane so is “rough”. It is found in the cells which make proteome for export .i.e hormones

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21
Q

What does the SER do?

A

No ribosomes- needed for synthesis and metabolism. Produces lipids

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22
Q

What does the Golgi body do?

A

Retrieve lipids and proteins from RER and sends them to either lysosomes or plasma membrane

23
Q

What to the lysosomes do?

A

A phospholipid bilateral and acid hydrolysed which are enzymes that break down macromolecules

24
Q

What is magnification?

A

How much larger a sample appears to be compared to its actual size

25
Q

What happens if you increase magnification?

A

You increase blurryness

26
Q

What is resolution?

A

The ability to distinguish two objects which are close together

27
Q

What happens if the resolution is higher?

A

You get more detail

28
Q

Why are electron microscopes better than light microscopes?

A

1) Light microscopes wavelength is too long (can’t distinguish 2 objects)
2) the resolution of an electronmicroscopes is higher

29
Q

What is an ultra microtome?

A

It slices cells so we can see them in the transmission microscope

30
Q

Compare TEM’s and SEM’s

A

TEM-internal view/2D/specimen sliced THIN/electrons shoot through the specimen/artefacts may occur
SEM-external view/3D/ short wavelength/electrons shoot and deflect off specimen/focused with electromagnets

31
Q

What is ultrastructure?

A

Things that you cannot see with a,ought microscope

32
Q

What is ultracentrifugation?

A

Spinning cells round at a very high G to separate the components in the cell

33
Q

What is cell fractionation?

A

Smashing open the cell to look internally

34
Q

What is centrifugation?

A

Isolation that cell organelles that you want to separate

35
Q

What is homoginization?

A

The actual process of opening the cells

36
Q

Why is the solution homogenized in an isotonic solution?

A

-to stop osmotic damage, with a pH buffer to regulate pH and at an ice cold temperature to prevent enzyme damage

37
Q

What is morphology

A

A fancy word for the shape of the cell

38
Q

What is anucleic?

A

A cell with no nucleus like a bacterial cell

39
Q

What is a light microscope?

A

Used in cells/long wavelength so poor resolution/look at specimen directly/specimen dead or alive/specimen easy to prepare

40
Q

What is mitosis?

A

The division of cells that result in each of the daughter cells having an exact copy of DNA of the parent cell

41
Q

What are the stages of mitosis?

A
Interphase
Prophase
Metaphase
Anaphase
Telophase
Cytokinesis
42
Q

What happens in interphase?

A

DNA replicates and is joined at the centromere

43
Q

What happens in prophase?

A

Centrioles move to opposite poles and spindle fibres develop which go from pole to pole

44
Q

What happens in metaphase?

A

Chromosomes seem to be made of 2 chromatids which are joined at the centromere. The chromatids move along the spindle apparatus and line up at the equator of the cell

45
Q

What happens in anaphase

A

The centromeres divided in 2 and the spindle fibre pull the chromatids apart. Energy for this is provided by the mitochondria

46
Q

What happens in telophase/cytokinesis?

A

Chromosomes reach their respective poles and become longer. The spindle fibres disintegrate and the nuclear envelope and nucleus reform.
Cytoplasm divides

47
Q

Why is mitosis important?

A

Growth
Repair
Reproduction

48
Q

How do you calculate mitotic index?

A

MI= No. Of cells with condensed chromosomes/total no. Of cells

X100

49
Q

What are oncogenes?

A

Proteins which switch off the process of cell Division

50
Q

What are pro oncogens?

A

Proteins which switch on the process of cell division

51
Q

What is begin?

A

Not spreading/not harmful

This is what most tumours are however the pay can compress and cause damage to surrounding tissue

52
Q

What is malignant?

A

Spreading/developed and are dangerous. Some tumours spread from their site of origin.they develop their own blood and lymph supply–>this transports the malignant cells SO THE TUMOUR SPREADS!!!!!:(:(:(

53
Q

How can you treat cancer?

A

You kill the cells by blocking part of the cell Cole- this disrupts the cell divisor so cancer growth ceases.
(Preventing DNA replication)