Chapter 28: Prokaryotes Flashcards
Microfossils
Fossilised forms of microscopic life
Oldest are 3.5 billion years
First cells:
Oldest: 3.5 billion years old
Microfossils - indicate prokaryotes were first cells
Stromatolites
Combination of sedimentary deposits and precipitated material held in place by cyanobacteria.
2.7 billion years old.
Do domains of prokaryotes include:
Bacteria + Archaea
Bacteria & Archaea (what are the differences?)
Plasma membrane
Cell Wall
DNA replication
Gene Expression
Archaea (habitat)
Extremophiles
Live in hot springs (extreme env.)
Prokaryotes (habitat)
Live everywhere eukaryotes do
Also able to thrive in places no eukaryote could live
Prokaryotes (differ from eukaryotes)
They are:
Unicellular
Cell size vary
Chromosomes - no membrane bounded nuclei. Single circular chromosome made of DNA
Cell division & genetic recombination - binary fission
Internal compartmentalisation (none)
Flagella - simple; single fibre of protein flagellin
Metabolic diversity
Prokaryotic Taxonomy (characteristics once used to classify prokaryotes)
- Photosynthetic or non
- Motile/nonmotile
- Unicellular, colony forming or filamentous
- Formation of spores or division by transverse binary fission
- Importance as human pathogens or not
Prokaryotic cell wall:
Complex
Many layers
Peptidoglycan (polymer unique to bacteria)
Two types of bacteria:
Can be identified using staining process:
Gram stain
Gram Stain
Gram-positive
Gram-negative
Gram-positive bacteria
Have thicker peptidoglycan wall
Stain purple colour
Gram-negative bacteria
More common
Contain less peptidoglycan
Do not retain purple coloured dye
Prokaryotic cell structure (3 basic shapes):
Bacillus - rod
Coccus - spherical
Spirillum - helical
Cell Surface Structures
Bacterial cell walls: peptidoglycan
Archaea cell walls: pseudomurein
Capsule
Present in some bacteria - additional gelatinous layer
Allow bacteria adhere to smooth surfaces & develop into structures: ‘biofilm’
Capsule inhibits human protective response and antimicrobial agents
S-layer
Present in some bacteria + archaea
An additional protein or glycoprotein layer forming rigid paracrystalline surface - ‘S-layer’
Endospores
Some prokaryotes able to form endospores
Develop a thick wall around genome and a small portion of cytoplasm
Resistant to environmental stress, esp. heat
Prokaryotes (internal)
Internal membranes: complex
Nucleoid region: lack nuclei, only containing single circular chromosome. DNA located in nucleoid
Ribosomes: smaller than eukaryotes, differ in protein & RNA content
Prokaryotic Metabolism
Acquire carbon & energy in four different ways:
Autotrophs (photo/chemolitho - autotrophs) and Heterotrophs (photo/chemo - heterotrophs)
Autotrophs - obtain carbon from inorganic CO2
Photoautotrophs - energy from the Sun
Chemolithoautotrophs - obtain energy from oxidizing inorganic substances
e.g. nitrifiers
Nitrifiers
Chemolithoautotrophs
Oxidize ammonia or nitrate to obtain energy, producing nitrate
Nitrate taken up by plants
Process called nitrification
Heterotrophs - from organic molecules
Photoheterotrophs: light is used as energy source but obtain organic carbon made from other organisms
Chemoheterotrophs: obtain both carbon and energy from organic molecules
e.g. human beings
Symbiosis
Ecological relationship between different species living in direct contact with each other
Mutualism
Both parties benefit
e.g. nitrogen fixing bacteria on plant roots
Commensalism
One organism benefits and the other is unharmed/unaffected
Parasitism
One organism benefits and the other is harmed
Sexually transmitted diseases
Mostly are bacterial: STDs Gonorrhea Syphilis Chlamydia("silent STD" - no symptoms until infection established)
Beneficial prokaryotes
Decomposers (carried out by prokaryotes and fungi) - release a dead organism’s chemicals to the environment
Fixation
Nitrogen fixer reduce N2 to NH3
three associations
Anabaena - in aquatic environments, nitrogen carried out by these cyanobacteria
Rhizobium - in plant roots in soils
Frankia - in plant roots in soil
Bioremediation
Bacteria used for this process:
removal of pollutants from water, air and soil
Current classification of prokaryotes is based on:
sequencing of proteins, DNA and RNA
Prokaryotic DNA is located:
nucleoid region
Not a characteristic of ALL prokaryotes:
cell walls constructed of peptidoglycan.