Chapter 25: DNA Metabolism

Question 1

Dna replication is considered

Answer 1

Semiconsrvative replication

Question 2

semiconservative repl

Question 3

Replication forks

Question 4

1 in euk multiple in eukaryotes

Question 5

Origin

Answer 5

Location where replication loops are inittated

Question 6

Where do origin sites typically happen?

Answer 6

A=T rich regions

Question 7

Denaturation mampping

Answer 7

denaturation of sequences unusually rich A=T bp to provide landmarks along the DNA molecule

Question 8

What does denaturation mapping generate

Answer 8

single stranded bubbles

Question 9

What direction are dyna synthesis created in?

Answer 9

5’ to 3’

Question 10

Ozakai fragments

Answer 10

Short DNA fragments that are created in replication of one of the enw DNA strands

Question 11

How are Okazaki fragments spliced togteher?

Answer 11

Via DNA ligase

Question 12

Leading strand

Answer 12

5’ to 3’ synthesis same direction as the replication fork

synthesized continuously

Question 13

Lagging strand

Answer 13

5’ to 3’ synthesis opposite direction that the replication fork moves

synthesized discountiosuly

Question 14

Ozaki fragments aid in what

Answer 14

the discontinuously synthesized lagging strand

Question 15

Nucleases

Answer 15

enzymes that degrade nucleic acids

Question 16

DNases

Answer 16

specifically degrade DNA

Question 17

Exonucleases

Answer 17

degrade nuekeic acids from one end of the molecules

opperate in one direction

Question 18

Endonucleases

Answer 18

degrade nucleic acids at specific interal sites in the molecules

Question 19

DNA olymerases

Answer 19

cenzymes that syntehsize DNA

Question 20

How many DNA polymerase do E. coli cells have?

Answer 20

5 DNA polymerases including DNA polymerase 1

Question 21

DNMP vs DNTP

Answer 21

deoxynucleo

Question 22

What reaction is DNA polymerase reaction?

Answer 22

phopshoryl group transfer

Question 23

slide 13

Question 26

Primer

Answer 26

A strand segment with a free 3’OH group to which a nucleotide can be added

Question 30

Insertion sire

Answer 30

Active site where the incoming nucleotide binds

Question 31

Post insertion site

Answer 31

Part of the active site where the new base pair resides when the polymerase slides forward

Question 32

Processiviity

Answer 32

number of nucleotides added before a polymerase dissociates

Question 33

DNA polymerase very greatly in processivitiy meaning

Answer 33

ranges from a few nucleotides to thousdans

Question 34

Post insertion site serves as

Answer 34

a checkpoint/error catcher for DNA replication

Question 35

Which polymerase is the most abundant in prokaryotes?

Answer 35

DNA Polymerase 1

Question 36

DNA polymerase 1

Answer 36

exonuclease activity
performs nick translation

Question 37

What’s nick translation?

Answer 37

A break or nick in the DNA moved along

Question 37

W