Chapter 2: Metabolic Blood Test and Analyte Detected (Overview) Flashcards
1
Q
A) Nelson- somogyi method
~~~>GLUCOSE & Reducing sugar
B) Dubowski reaction/Condensation mtd/Orthotoluidine~~~>GLUCOSE
•(+) Bluish green (620-630nm)
C) Van Handel Zilversmit method~~>TRIGLYCERIDES
D) Lieberman Burchard reaction~~>CHOLESTEROL
(1st step mtd:(+)Green)
• Abell- Kendall method (Reference method)
E) BIURET REACTION~~~>PROTEIN
• (+) VIOLET
F) PARFENTJEV REACTION~~~>FIBRINOGEN
G) JAFFE REACTION~~~>CREATININE
H) Fearon reaction/Diacetylmonoxime(DAM)~>UREA
•(+) YELLOW
I) Berthelot reaction ~~~>UREA
•(+) INDOPHENOL BLUE
J) CARAWAY METHOD/Phospotungstic Acid
~~~> URIC ACID ; 650(660nm)-700nm
•(+) TUNGSTEN BLUE
A
K) CONWAY~~~>AMMONIA L) LDH •Wacker method ~~>(Forward)= LACTATE—>PYRUVATE [“FLaP”] • Wrobleuski La due ~~>(Reverses)= PYRUVATE—> LACTATE [“RePYL”] M) CK • TANZER-GILVARG (Forward)~~>[“TGiF”] • Oliver- Rosalki/Rosalki-Hess (Reverse) N) ALP • BOWERS- COMB METHOD (Reference method) • Bessey- Lowry- Brock O) SZASZ METHOD ~~~> GGT P) ELLMAN METHOD ~~~> CHOLINESTERASE Q)DIXON&PURDON,CAMPBELL,BELFIELD &GOLDBERG METHOD~~~> 5-NUCLEOTIDASE R) Karmen method ~~~> AST S) Reitman- Frankel method ~~~>AST&ALT T) SCHALES-SCHALES~~~> CHLORIDE U) Clark- Collip~~~> CALCIUM V) Titan Yellow method ~~~> MAGNESIUM W) Fiske- Subbarow~~~> PHOSPHATE X) Kober reaction————,Handbook~~>ESTROGEN Y) Porter- SilberReaction >Of~~>CORTICOSTEROID Z) Zimmerman method \_\_\_’Pear~>17-KETOSTEROID
