Chapter 2 - Enzymes Flashcards
1
Q
What is Km
A
The substrate concentration at half Vmax
2
Q
What is Kcat?
A
The ‘turnover’ rate
The number of substrate molecules turned into products
Vmax = [E]kcat
3
Q
Write out the lineweaver Burke equation
A
1/v= (Km/Vmax)(1/[S])+(1/Vmax)
Y = mx + b
4
Q
Name four types of reversible inhibition
A
Compétitive
Non competitive
Mixed
Uncompetitive
5
Q
Competitive inhibition:
Where is binding site?
Impact on Km?
Impact on vmax?
A
Binding site: Active site
Km: increases
Vmax: same
6
Q
Non competitive inhibition:
Binding site, km, vmax
A
Binding site: allosteric site
Km: same
Vmax: decreases
7
Q
Mixed: where is binding site? Impact on Km and vmax?
A
Binding site: allosteric
Km: increases or decreases
Vmax decreases
8
Q
Uncompetitive: binding site? Impact on km and vmax?
A
Allosteric site
Km and vmax decrease
