Chapter 2: Basic Components Of Living Systems Flashcards
Magnification
Describes the enlargement of a specimen under a microscope
Resolution
The smallest distance that 2 objects can be apart while still appearing as 2 objects
Light microscope
- light sent through specimen and image that is magnified by glass lenses
- cheap
- easy to use
- can study living tissues and cells
- 2D image
- max resolution 200nm
- max magnification ×2000
Laser scanning microscope
- uses a high power beam of light to create an image
- passes over every point in specimen and creates image over time
- flourrscent dye added to cell
- more expensive
- high resolution
- 3D
Electron microscopes
TEM - Highest resolution of 0.5nm SEM - Resolution of 3-10nm - 3D image
- magnification of over ×500,000
- blak and white images
- requires a vacuum
Dry mount
Solid specimens sectioned and placed on slide with cover slip on top
Wet mount
Specimens suspended in a liquid (eg. Water, immersion oil)
Cover slip placed from an angle
Squash slides
First wet mount
Then a lens tissue is used to gently press down the cover slip
Smear slide
Edge of a slide is used to smear sample to form a smooth thin coating on another slide
Structure of cyanobacteria (photoautotrophs)
Circular DNA
Ribosomes
Cell Wall
Microtubules
Part of cytoskeleton and require energy to move- function can be prevented by a respiratory inhibitor
Responsible for moving chromosomes from equator to poles of the cell- make up spindle fibres