Chapter 2

Question 1

How do you determine the magnification when using a microscope?

Answer 1

Objective x ocular

Question 2

What is resolving power?

Answer 2

The ability to distinguish b/t two close objects

Question 3

d=0.5λ/ n sin θ

Answer 3

Numerical aperture

Question 4

What does λ stand for in the numerical aperture?

Answer 4

amount of light that must pass b/t two objects for them to be seen as separate

Question 5

What does “n” stand for in the numerical aperture?

Answer 5

Light bending properties (refractile index)

Question 6

What is the refractile index of air? Oil?

Answer 6

n Air = 1.0

n Oil = 1.5

Question 7

Light microscopes

Answer 7

1000-2000x magnification

use immersion oil, and blue light

Question 8

Different types of light microscopes?

Answer 8

Bright field, dark-field, phase-contrast and fluorescence

Question 9

Electron microscopes

Answer 9

Beam of electrons in a vacuum, magnification over 100,000X, resolving power = 0.005nm

Question 10

Different types of electron microscopes?

Answer 10

Transmission and Scanning