Chapter 2 Flashcards
What is the cell theory?
Cell Theory
- All organisms are composed of one or more cells.
● Prokaryotes - one cell (unicellular- carry out activities necessary for life)
● Eukaryotes - one or more cells (uni or multicellular- the activities of life are divided among numerous types of specialized cells)
- The cell is the basic structural and functional unit of all living organisms. (cannot separate mitochondria or other individual parts)
- Cells arise only from the division of preexisting cells (do not occur spontaneously)
What two types of microscopes are used to see cells and the structures within them?
Light microscopes use light to illuminate the specimen
Electron microscopes use electrons to illuminate the specimen
How big are cells?
Most cells are too small to be seen by the unaided eye—ranging from about 0.5 μm (bacteria) to a few hundred micrometres (plant cells)
Largest known cell called Valonia Ventricosa (algae)
What is the plasma membrane and what are its main functions?
All cells are surrounded by the plasma membrane, a bilayer made of lipids with embedded protein molecules
- Plasma membrane - bilayer made of lipids w/ embedded protein molecules
- Plasma membrane consists of phospholipid bilayer - hydrophobic barrier to water-soluble
substances
● Selected water-soluble substances can penetrate cell membranes through the transport of protein
channels
● Selective transport of ions + water-soluble molecules maintain specialized internal
environments required for cellular life
● Amphipathic - hydrophobic tail, hydrophilic head
What are the differences between prokaryotic cells and eukaryotes?
Prokaryotic cell (characteristic of domains Bacteria and Archaea)
□ The nucleoid region has no boundary membrane (no nucleus)
□ Many Bacteria and Archaea species contain few if any internal membranes
Eukaryotes (domain, or sub-domain?, Eukarya)
□ Have a membrane-bound compartment called the nucleus
□ Cytoplasm typically contains extensive membrane systems that form organelles
What type of cell is single-cellular but still eukaryotic?
Protists
What are the characteristics of Eukaryotic cells?
The eukaryotes include animals, plants, fungi and protists
Eukaryotic cells have a membrane-bound compartment called the nucleus
► ”eu” = “true” or “well”; “karyon” = “kernel” or “nucleus”
► “pro” = “before”
The eukaryotic cytoplasm contains a system of membranous organelles, specialized to carry out functions of energy metabolism and molecular synthesis, storage, and transport
The cytosol participates in energy metabolism and molecular synthesis and functions in support and motility
Photosynthesis occurs in the chloroplasts
What are the characteristics of prokaryotic cells?
Three shapes are common among bacterial prokaryotes: spherical, rodlike, and spiral
Information from DNA has copied into messenger RNA (mRNA) molecules and carried to ribosomes in the cytoplasm, which assemble amino acids into proteins
The plasma membrane is typically surrounded by a more rigid bilayer external cell wall coated with polysaccharides (glycocalyx)- assume they all have a cell wall, it is a slime layer; when it is firmly attached, it is a capsule
The plasma membrane contains molecular systems that metabolize food molecules (or light energy) into the chemical energy of ATP (cellular respiration occurs in prokaryotes)
Many bacteria and archaeans move using long flagella—the bacterial flagellum rotates in a socket and pushes the cell through a liquid medium
How do the plasma membrane and cell wall relate?
The eukaryotic plasma membrane is responsible for many functions involving multiple types of integral membrane proteins:
► Channel proteins transport substances in and out of cells
► Receptors recognize and bind specific signal molecules in the cellular environment and trigger internal responses
► Immune system proteins label cells as “self”
► Membranes and membrane proteins covered
A supportive cell wall surrounds the plasma membrane of fungal, plant, and many protist cells
What are Eukaryotic Ribosomes?
consist of large and small subunits
□ Designated as “80S” ribosomes (prokaryotic, mitochondrial and chloroplast ribosomes are referred to as “70S”)
Some ribosomes are freely suspended in the cytosol; others are attached to the membrane of the endoplasmic reticulum
Proteins made on free ribosomes may remain in the cytosol, pass into the nucleus, or become parts of mitochondria, chloroplasts, cytoskeleton, or other cytoplasmic structures
Proteins made on ribosomes attached to the endoplasmic reticulum (ER) follow a special path to other organelles in the cell
What is magnification?
The ratio of the object as viewed to its real size (e.g., 1200X, 400:1)
What is resolution?
The minimum distance that two points in the specimen can be separated and still be seen as two points
Depends primarily on the wavelength of light or electrons used to illuminate the specimen
Why are cells so small?
Cell size is limited by the surface area-to-volume ratio
The volume of a cell determines the amount of chemical activity that can take place within the cell.
Surface area determines the number of substances that can be exchanged between a cell and the outside environment
When a cell gets bigger, does its surface area-to-volume ratio go up or down? Explain
● Bigger cell = more capacity to undertake biochem rxns
- Surface area determines the number of substances that can be exchanged b/w cell and outside environment
● Bigger SA = more space to move things in/out
- Doubling diameter of cell = increase SA by 4x = incrase volume by 8x
- Increase size of cell = V + SA don’t increase at same rate = ratio so unproportionate that SA won’t be able to support the biochem means that V can manage
What is the difference between the word prokaryote and prokaryotic cell?
- Prokaryote - describes a unique group of evolutionarily related organisms
- Prokaryotic cell - refers to a particular cell architecture
● Lacks nucleus & not to single group of organisms
In what way is scanning electron microscopy different from transmission electron microscopy?
Scanning e- microscopy - a beam of e- scanned across a whole cell or organism
● e- excited on the specimen surface is converted to a 3D appearing image
Transmission e- microscopy - a beam of e- focused on a thin section of specimen in a vacuum
● e- that pass through from the image
● Structures that scatter e- appear dark
● Used primarily to examine structures w/in cells
● Staining + fixing methods used to highlight structures of interest
What is featured in the internal organization of the cell?
Central region
Genes
Cytoplasm
Cytoskelton
Cytosol
What is the central region?
Contains DNA molecules, which store hereditary information (genes)
Genes?
segments of DNA that code for individual proteins
Cytoplasm?
Between the plasma membrane and the central region, contains the cytosol and cytoskeleton
Cytosol?
Aqueous solution containing ions, various organic molecules, and organelles
Cytoskeleton?
Maintains cell shape and plays key roles in cell division, chromosome segregation, and transportation within the cell
What is the nucleus?
Separated from the cytoplasm by the nuclear envelope, which consists of two membranes
One membrane is layered just inside the other and separated by a narrow aqueous space (2 phospholipid bilayers)
Nuclear pore complexes embedded in the nuclear envelope regulate the transport of proteins and RNA molecules between the nucleus and cytoplasm (messenger RNA)
A channel through the nuclear pore complex, a nuclear pore, is the path for the assisted exchange of large molecules with the cytoplasm
What is the space inside the nucleus is mostly occupied by?
chromatin (a substance that is produced after combining), a combination of DNA and proteins
Chromatin can be more or less condensed at different times, giving chromosomes a different appearance
In eukaryotes, each DNA molecule is linear, and associates with proteins into chromosomes?
True
Does a eukaryotic nucleus contain one or more nucleoli, formed around the genes coding for rRNA molecules (transcription) of ribosomes (several different rRNA that go into forming ribosomes)?
True
How are ribosomal subunits formed and where do they exit?
Ribosomal subunits are formed in nucleoli and exit the nucleus through nuclear pore complexes. In the cytoplasm, they combine with other rRNA molecules and proteins to form ribosomes
What are ribosomes are the site of? What occurs?
Ribosomes are the site of translation, where mRNA is converted into proteins. Expression of genes is carefully controlled, according with the function of each cell
More protein = more ribosomes = nucleoli will be larger?
True
3 most common shapes of prokaryotes?
Spherical, rodlike, spiral
Eukayotic cells have an …. system that divides the cell into functional and structural compartments
Endomembrane
collection of interrelated internal membranous sacs that divide a cell into
functional and structural compartments
What are the compnent of the endomembrane system?
The membranes may be connected directly (physically) or indirectly by vesicles, small membrane-bound sacs that transfer substances between parts of the system
ER
Nuclear envelope
Golgi
Lysosomes
Vacuoles
Plasma membrane
What is the ER?
Endoplasmic reticulum (ER)
→ Extensive interconnected network (reticulum) of membranous channels and
cisternae
→ Each cisterna is formed by a single membrane that surrounds an enclosed space (ER lumen)
ER occurs in two forms: rough ER and smooth ER
What is the difference between the rough ER and the Smooth ER
Rough ER has many ribosomes attached to its outer surface
Proteins that are made on ribosomes attached to the ER enter the ER lumen or become integrated into the ER membrane
Rough ER contains ribosomes, proteins, lumen, carbohydrates
Proteins are then delivered to other regions of the cell (Golgi complex) using small vesicles that form from the ER
Smooth ER membranes have no ribosomes attached to their surfaces (no porteins being made)
synthesizes lipids that become part of cell membranes, stores Ca2+ ions and cell signaling
In the liver, smooth ER converts drugs, poisons, and toxic by-products into substances that can be tolerated or more easily and removed from the bod
What is the golgi complex?
The Golgi complex consists of a stack of flattened, membranous sacs (cisternae)
Responsible for final modification, sorting, + distribution of proteins + lipids
Proteins enter cis face through vesicles and modified porteins exit by trans face to plasma membrane (from which they leave out of cell by exocytosis)
Add carbs, lipids + functional groups (can cut but NOT add amino acids)
Exocytosis?
A secretory vesicle fuses with the okasma membrance, releasing the vesicle contets to the cell exterior. The vesicle membrane becomes a part of the plasma mebrane
Can be used to deliver plasma membrane proteins to the plasma membrane.
What is the relationship between the golgi complex and exocytosis?
The Golgi complex “sorts” proteins to ensure they are delivered to their final destination. (some proteins are taken back).
Proteins to be secreted from the cell are transported to the plasma membrane in secretory vesicles, which release their contents to the exterior by exocytosis
The membrane of the vesicle fuses with the plasma membrane and becomes part of the plasma membrane
Endocyosis?
Materials from the cell exterior are enclosed in a segment of plasma membrane that pockets inward and pinches off as an endocytic vesicle
Vesicles also form by the reverse process, endocytosis, which brings molecules into the cell from the exterior
The plasma membrane forms a pocket, which bulges inward and pinches off into the cytoplasm as an endocytic vesicle
Endocytic vesicles carry materials to the Golgi complex or other destinations such as lysosomes
What are lysosomes?
Lysosomes are small, membrane-bound compartments containing hydrolytic enzymes that digest complex molecules
► Cells recycle the constituents of these molecules
Found in animals + plants (eukaryotes)
Formed by budding from golgi complex
pH in lysosome is acidic (5) < pH of cytosol (7.2)
- Lysosomes do not digest endocytic vesicles
● Secretory vesicles carry materials out
● Endocytic vesicles carry materials in
In lysosomal storage diseases, one of the hydrolytic enzymes normally found in the lysosome is absent (cannot break down and recycle things).
Vesicle traffic and the endomembrane system porcess
- Proteins made by ER
ribosomes enter ek membranes
or the space inside ER cisternae.
Chemical modification of some
proteins begins. Membrane lipids
are also made in the ER. - Vesicles bud from
the ER membrane and
then transport unfinished
proteins and lipids to the
Golgi complex. - Protein and lipid
modification is completed
in the Golgi complex, and
products are sorted into
vesicles that bud from
the complex. - Secretory vesicles budding
from the Golgi membranes
transport finished products to the
plasma membrane. The products
are released by exocytosis. Other
vesicles remain in storage in
the cytoplasm. - Lysosomes budding from the
Golgi membranes contain
hydrolytic enzymes that digest
damaged organelles or the
contents of endocytic vesicles that
fuse with them. Endocytic vesicles
form at the plasma membrane
and move into the cytoplasm.
What is a nucleoid?
Nucleoid - central region of a prokaryotic cell with no boundary membrane separating it from the
cytoplasm (containing cytosol + cytoskeleton), where DNA replication and RNA transcription occur
Genetic material of archaea and bacteria in nucleoid
Some bacteria and archaeans have internal membranes. How are these internal membranes formed?
Endomembrane system - a collection of internal membranes that divide cell into structural +
functional regions
● Include golgi complex, ER, + nuclear envelope
- Internal membranes formed infolding of plasma membrane
● Most often the sites of photosynthetic + respiratory e- transport chains
Basic structure of prokaryotic cell
Plasma membrane surrounded by cell wall
- Cell wall - provides rigidity to prokaryotic cells
- Cell wall coated w/ glycocalyx - a carbohydrate coat covering the cell surface
● Glycocalyx diffused + loosely associated w/ cells = slime layer
● Glycocalyx gelatinous + more firmly attached to cells = capsule
● Helps prokaryotic cells from physical damage + desiccation
● can enable cell to attach to surfaces s/a other prokaryotic cells (forming a colony),
eukaryotic cells (streptococcus attaching to lung cells), or non-living substrate (rock)