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Microbiology > Chapter 19 Enterobacteriaceae > Flashcards

Chapter 19 Enterobacteriaceae Flashcards

Exam 3

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Biology 101 flashcards
1
Q

What are the general characteristics of Enterobacteriaceae?

A

Ferment glucose
Reduce nitrate to nitrites
Oxidase negative

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1
Q

What is the morphology of Enterobacteriaceae

A

Gram-negative coccobacilli or straight rods

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2
Q

True or false. Enterobacteriaceae is facultatively anaerobic

A

True

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3
Q

What is the macroscopic morphology of Enterobacteriaceae

A

Large moist, gray colonies

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4
Q

Bile salts and crystal violet inhibit gram-positive
It is selective and differential
Lactose fermentation is differential
What agar plate is this?

A

MacConkey MAC agar

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5
Q

Selective and differential
Methylene blue inhibits gram-positive
Lactose and sucrose fermentation is differential
What agar plate is this?

A

Eosin-methylene blue (EMB) agar

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6
Q

Selective and differential
Bile salts inhibit gram-positive, some gram-negative
Lactose and sucrose fermentation is differential
What agar plate is this?

A

Hektoen enteric (HE)

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7
Q

Green to blue color with H2S gas creating a black ppt is what type of enterobacteria

A

Salmonella on HE agar

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8
Q

Green to blue green color is what type of enterobacteria

A

Shigella on HE agar plate

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9
Q

Selective and differential
Has Sodium Deoxycholate
What type of agar plate is this?

A

Xylose lysine deoxycholate (XLD) agar

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10
Q

Sodium Deoxycholate does what

A

Inhibits gram-positive and some gram-negative

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11
Q

On the XLD agar plates, Escherichia coli and Citrobacter will produce what kind of colonies?

A

Yellow colonies

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12
Q

Yellow colonies on the XLD agar means that fermenters of those not producing what?

A

lysine decarboxylase

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13
Q

On XLD agar what kind of colonies will shigella produced?

A

Colorless or red colonies

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14
Q

On XLD agar what colonies will Salmonella will produced

A

Red colonies with black centers

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15
Q

What does adherence mean?

A

Prevent being removed from tissues

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16
Q

Enterobacteriaceae has what type of antigens?

A

O antigen
H antigen
K antigen

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17
Q

Where is the O antigen located at?

A

In the cell wall and it is the heat stable antigen

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18
Q

H antigen is the what type of antigen?

A

Flagellar and it is heat stable

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19
Q

K antigen is what type of antigen

A

Capsular antigen which is heat labile

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20
Q

Dry pink colonies
Beta-hemolysis on blood agar plate (BAP)
Motile
Possess adhesive fimbriae and sex pili
Possess O, H, K antigen
is what type of Enterobacteriaceae

A

E.coli

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21
Q

What is the most common cause of UTIs?

A

Uropathogenic E.coli

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22
Q

Uropathogenic E.coli usually originate from where?

A

In the large intestine as normal flora

23
Q

What can cytolysins do

A

Kill immune cells and inhibit phagocytosis

24
Q

Klebsiella belong to what tribe

A

Klebsielleae

25
Q

Where can the tribe members be found at

A

In the intestinal tract of humans and animals, in soil, water, and plants

26
Q

Klebsiella pneimoniae can be in the

A

lower respiratory tract infection of hospitalized patients

27
Q

What is the virulence factor of klebsiella pneumoniae

A

polysaccharide capsule

28
Q

A documented pathogen causing meningitis and bacteremia in neonates from powdered infant formula

A

Cronobacter sakazakii

29
Q

Opportunistic pathogens associated with outbreaks in health care settings

A

serratia species

30
Q

What species is this of Serratia
Pink-to-red pigment when growing at room temp
Usually most clinically relevant
Nosocomial infections of the Urinary tract, respiratory tract, and bacteremia

A

S. marcescens

31
Q

What type of species of Serratia is this?
Dirty, musty odor resembling that of rotten potatoes

A

S. odorifera

32
Q

What type of bacteria is this?
Linked to gastroenteritis and occasionally isolated from stool cultures

A

Hafnia alvei

33
Q

P. mirabilis and P. vulgaris generally produce swarming on what type of laboratory media. What kind of smell is produce

A

Burnt chocolate odor

34
Q

What Morganella species can cause UTI species, is a possible agent of diarrheal disease and cause of neonatal sepsis

A

M. morganii

35
Q

P.rettgeri can cause what type of outbreaks

A

Urinary tract pathogen and occasionally nosocomial outbreaks

36
Q

What is the most common citrobacter species

A

C. freundii

37
Q

What are three major clinical types of plesiomonas that cause gastroenteritis

A

Watery or secretory diarrhea
Chronic disease that lasts from 14 days to 2-3 months
Resembles colitis

38
Q

True of false- Plesiomonas is oxidase positive

A

True

39
Q

Is direct microscopic examination helpful in differentiating the species of enterobacteriaceae?

A

No it is not very helpful

40
Q
A
41
Q

Characterized by prolonged fever and intermittent bacteremia

A

Nontyphoidal bacteremia

42
Q

This type of bacteria appear clear, colorless, nonlactose-fermenting on MAC and on HE or XLD and does not produce H2S

A

Shigella

43
Q

Lack H antigens, has K antigen and O antigens are separated by serologic grouping A through D

A

Shigella

44
Q

Most serious infection (developing countries) is what type of shigella species

A

S. dysenteriaae group A

45
Q

Second most common isolate in the United States is what type of shigella species

A

S. flexneri Group B

46
Q

This type of shigella is more common in developing countries

A

S. boydii (Group C)

47
Q

This type of shigella is most common isolate in the United States

A

S. sonnei Group D

48
Q

Shigella dysentery invade what type of tissues?

A

Colonic tissues

49
Q

What is the causative agent of plague?

A

Y. pestis

50
Q

Gram-negative coccobacilli resembling a safety pin appearance (bipolar staining)

A

Yersinia pestis

51
Q

This type of yersinia can be acquired from contact with swine, cats, and dogs.

A

Yersinia enterocolytica

52
Q

Gram-negative coccobacilli appearance (bipolar staining)

A

Y. enterocolytica

53
Q

This type of yersinia is the primary pathogen of rodents, farm animals, and birds

A

Yersinia pseudotuberculosis

54
Q

This type of media contains antimicrobials or chemicals

A

selective media

55
Q

This media allows differentiation of bacterial species based on colony morphology

A

Differential media

56
Q
A

Microbiology (5 decks)

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