Chapter 19, 23, & 25 Flashcards
Are the following rules the correct puncture procedure for collecting capillary blood:
- Puncture away from midline of the finger or heel.
- Do not puncture parallel to the groves or lines of the fingertip.
- Make a quick stab with the lancet no greater than 2 mm deep on the side of the finger (off-center).
True or False?
TRUE
Fungi that appear refractile, or reflective, fragments of fungal elements are found on what part of the body?
Skin and nails
How would you move or transport microscopes?
Holding the arm and base of the microscope
When should specimens be labeled?
Before leaving the patient
Gram positive bacteria stain what color?
Deep blue or purple
When using the ocular lenses, how would you calculate the total magnification of a specimen?
Multiply ocular magnification power times objective magnification power
When using the coarse adjustment knob, how many inches is objective lens away from the object?
1/4 inch
The addition of one drop of 2% acetic acid to urine sediment will disintegrate what urine sediment cell?
Red Blood Cells (Erythrocytes)
What method are prepared from clinical specimens or from culture media spread across a glass slide for microscopic examination?
Smear
As hyphae continue to grow and branch, they develop into a mat of growth called?
Mycelium (pl. mycelia)
Which leukocyte is associated with inflammatory disorders and certain leukemias?
Basophil
What takes place when antibodies bind with specific antigens depending on a close three-dimensional fit?
Antigen-antibody reaction
What anticoagulant does the green top use for chemistry?
Heparin
What type of urinalysis includes the examination of physical characteristics, chemical characteristics, and microscopic structures in the sediment?
Routine urinalysis
What does dark orange urine indicate the presence of?
Pyridium
When performing a finger or heel puncture, the first drop should be wiped away to avoid what?
Dilution of the specimen with tissue fluids.
What test is an accurate, 2-minute disposable test designed to detect the presence of infectious mononucleosis antibodies in serum, plasma, or whole blood?
Monospot test
What Standard Form is the “Laboratory Report Display”?
SF 545
What is defined as the density of a solution compared to an equal volume of distilled water?
Specific gravity
What is the term used for the volume of erythrocytes expressed as a percentage of the volume of whole blood in a sample?
Hematocrit
Where are most capillary blood collected from an adult?
Middle or ring finger
What is the term used for organisms that do not reproduce in the presence of oxygen?
Anaerobes
Reactive specimens for Rapid Plasma Reagin (RPR) tests look like? Non-reactive?
Reactive = black clumps against white backgroundNon-reactive = even, light gray color
In manual method for determining hemoglobin, blood is mixed with ______________. This process hemolyzes, or destroys red cells (erythrocytes), disrupting the integrity of erythrocytes membranes and causing the release of hemoglobin, which is converted to a brownish-colored solution.
Cyanomethoglobin
What part of the compound microscope illumination system is located on the condenser to control the amount and angle of light rays that will pass to the specimen and lens, which affects the overall resolution, or ability to observe and interpret a sample?
Iris Diaphragm
Which study within the CBC often provides the most helpful information in determining the severity and type of infection?
White Blood Cell (WBC) Differential Count
What is the approximate magnification for high-power objective lens?
40x
What sample medical conditions are determined for (a) below and (b) above hematocrit determinations?
(a) below = anemia (b) above = dehydration
Where are capillary blood collected from a newborn?
Heel or great toe
What is widely used to detect and measure the presence of antigen-antibody reactions?
Agglutination tests
The Rapid Plasma Reagin (RPR) kit a specific test to diagnose what?
Syphilis
If any arm veins cannot be used due to interference such as IV fluid or mastectomy, who must be consulted to use hand or foot veins?
Supervisor
Do you let the alcohol pad or povidone-iodine solution dry after cleansing the fingertip?
Yes
Which type of toxin is produced by bacteria, found outside the cell wall, and is considered highly poisonous and associated with septic shock?
Exotoxin
What bacilli bacteria causes Gas gangrene, Tetanus, and Botulism in a Gram-positive stain?
Clostridium tetani
What test consists of specially prepared, stable sheep or horse erythrocyte antigen (dyed) and guinea pig antigen on a disposable slide?
Monospot Test
What is the term used for organisms that reproduces in the presence of oxygen?
Aerobes
Hat is the term used for specific defensive proteins produced by the host in response to the presence of an antigen and its primary function is to combine with antigens in body defenses?
Antibodies
In Rapid Plasma Reagin (RPR) Card test method of syphilis detection, what specific antigen detects “Reagin”, a substance present in the serum of persons who are infected with syphilis or suffering from similar tissue damage?
Carbon-particle cardiolipin
What is the function of hemoglobin in the body?
Delivers and releases oxygen to the tissues and facilitate carbon dioxide excretion
Freshly passed urine is usually clear or transparent. True or False?
TRUE
What anticoagulant does the light blue top use for coagulation studies?
Sodium citrate
It is important to obtain a focus with the low-power first, then change to the higher objective. Tue or False?
TRUE
When performing a WBC Differential, which cell has a lilac-colored cytoplasm and a dark or reddish purple nucleus that may be oval, horseshoe or “S”-shaped, or segmented (lobulated)?
Neutrophil
What are the normal hemoglobin determination values for (a) women and (b) men?
(a) women = 12-16 (b) men = 14-18 grams per 100 ml blood
Which temperature requirement category are bacteria that reproduces best at body temperature (35 degrees C) and are the primary pathogens to man?
Mesophilic
Which part of the compound microscope framework is the structure on which the microscope rests?
Base
What method of blood collection is performed when a small quantity of blood is needed for testing as in the case of some pediatric blood draws and when access are limited on a severely burned patient or ICU patient?
Capillary Blood Collection Method
What urine sediment cell are derived from the linings of the genitourinary system?
Epithelial cells
Large numbers of casts in the urine is almost sure to be positive for?
Albumin
What are chlorophyll-free, heterotrophic (not self nourishing) of the same family of plants as algae and lichens; reproduced by spores that germinate into long filaments called hyphae?
Fungi
What is the specific concern that is enacted by Congress and is enforced via the Departments of Health and Human Services (HHS) detailing how protected health information (PHI) may be transmitted and released?
Health Insurance Portability and Accountability Act (HIPAA)
What does olive green to brown-black urine indicate the presence of?
Phenols
What is the condition referred to when WBC rises above normal values and occurs when systemic or local infections are present?
Leukocytosis
Which leukocyte helps respond by ingesting invading bacteria?
Neutrophil
Which neutrophil sub-classification is an older or intermediate neutrophil that started to elongate and has curved itself into a horseshoe, C or U-shaped and it’s sometimes called “stab” cell?
Neutrophilic Band
Which type of lens is responsible for the magnification and resolution of detail in a specimen, has a set of three lenses, and it is the lens nearest the specimen?
Objective lens
What is also known as the vacutainer method that is defined as the puncture of a vein for drawing blood in which the arm is considered the best site for obtaining blood?
Venipuncture
Which leukocyte is generally round, oval, or slightly indented, and the chromatin (a network of fibers within the nucleus) is lumpy and condensed at the periphery?
Lymphocyte
What is the normal color of urine?
Varies from straw to amber
What are the three categories for bacteria temperature requirements?
Psychrophilic, Mesophilic, and Thermophilic.
Which control knob is the smaller outer knob that when rotated, renders the image clear and well-defined?
Fine Control Knob
What type of strips consist of chemical impregnated absorbent pads attached to a plastic strip?
Urinalysis Reagent Strips
What are the four parts of the compound microscope framework?
Arm, stage, mechanical (movable) stage, and base.
Specimens that have been refrigerated must be allowed to return to room temperature prior to reagent testing, since some enzymatic reactions on the strips are temperature dependent. True or False?
TRUE
The following are ways bacteria is classified: Disease-producing ability; growth requirements; morphologic characteristic; toxins produced; and gram’s stain reaction. True or False?
TRUE
Which urine specimen is the most commonly (routine) used because of the ease of collection and convenience for the patient and considered the least valid specimen?
Random Urine Specimen
What are the normal values of hematocrit for a normal, healthy (a) female and (b) male?
(a) female = 37-47% (b) male = 42-52% of blood volume
What are the 5 items collected and documented upon creation of a treatment record at the point of accession?
G6PD, Sickle Cell, ABO/Rh (Blood Type), DNA, and current HIV.
What cocci bacteria causes Pneumonia in a Gram-positive stain?
Streptococcus pneumoniae
What is the term used for a substance that, when introduced into an individual’s body, is recognized as foreign to the body, and causes a detectable reaction?
Antigen
What are the four growth requirements for bacteria?
Temperature, oxygen, nutrition, and moisture.
What percent of Potassium hydroxide (KOH) is used to test for fungi?
10%
What part of the compound microscope contains openings into which objective lenses are fitted, and revolves objectives into desired position?
Revolving Nosepiece
What part of the compound microscope illumination system is located in the base providing a precise and steady source of light into the microscope?
Internal light source
What two methods of preservation are used to delay the decomposition of urine?
Refrigeration and preservatives (hydrochloric acid)
What part of the compound microscope is a tube that permits light to travel from the objective to the ocular lens?
Body Tube
What does red or red-brown urine indicate the presence of?
Blood
What is the term used to define bacteria that are spherical or round in shape?
Coccus (pl. cocci)
For most of the clinical laboratory examinations, what is considered the preferred method of obtaining blood specimens?
Venipuncture
Which color tube is the best choice for routine chemistry test?
Red top
How often would the phlebotomy work site equipment and furniture be disinfected?
Daily or as needed after patient care
What is the term used to define bacteria that is helical, spiral, or crockscrew-shaped?
Spirochetes (pl. spirilla)
What would you want to do with the urine during the 24-Hour urine collection?
Refrigerate
In some difficult draw situations, the tourniquet is sometimes left on until the last tube is filled. How long would you leave the tourniquet on?
No more than one minute
What is the normal daily urine volume for an adult?
600-2,000 ml
Which type of lens, also known as “eyepiece” is located on top of the body tube and has a magnification power of 10x?
Ocular Lenses
What is the function of leukocytes (White Blood Cells)?
To control various disease conditions
What are the two ways to perform a Complete Blood Count (CBC)?
Manually or automated hematology analyzers
What study consists of an examination of blood to determine the presence and the number of different types of white blood cells usually expressed in a percentage?
White Blood Cell Differential Count
This test consists of procedures by which antigens and reacting serum globulin antibodies may be measured qualitatively and quantitatively based on agglutination reactions between an antigen and a specific antibody?
Serology
How would you remove (a) dust and (b) oil from compound microscopes?
(a) Lint-free lens tissue (b) lens cleaning solution with lens tissue
Hemoglobin concentration below 7 indicates severe anemia and may require?
Blood transfusion
Which part of the compound microscope framework supports the magnification and focusing system and is the handle by which the microscope is carried?
Arm
What cocci bacteria causes Meningitis in a Gram-negative stain?
Neisseria meningitis
What cocci bacteria causes Gonorrhea in a Gram-negative stain?
Neisseria gonorrhoeae
Gram negative bacteria stain what color?
Deep pink
What is term used to define bacteria that are rod-shaped?
Bacillus (pl. bacilli)
What bacilli bacteria causes the Bubonic plague in a Gram-negative stain?
Yersinia (Pasteurella) pestis
What are some common superficial infections of the skin caused by fungi?
Athlete’s foot and ringworm of the scalp
What are the five types of White Blood Cells (WBCs) normally found in the circulating blood?
Neutrophils, Eosinophils, Basophils, Lymphocytes, and Monocytes.
What urine sediment cell is not normally found in urine?
Red Blood Cells (Erythrocytes)
What is the normal number of leukocytes per high-power filed seen on a microscopic examination?
0-3 leukocytes per high-power field
Which urine specimen is the first urine voided upon rising is considered the most ideal screening because it is usually concentrated?
First Morning Urine Specimen
What type of leukocyte compromises the largest percentage of cells in the circulating blood?
Neutrophil
The best urine specimen for screening purposes is that taken as?
First morning
Which leukocyte is associated with immune response and the body’s defense against viral infection?
Lymphocyte
In Gram’s stain, what color is the primary stain?
Crystal violet
White Blood Cell (WBC) count above 50,000 indicate?
Acute infection
What is the minimum file percentage of certain ship’s company is required to be enrolled in the Walking Blood Bank?
10%
What anticoagulant does the lavender top use for Complete Blood Count (CBC)?
EDTA
Which leukocyte is the largest of the normal White Blood Cells (WBC), controls microbial and fungal infections, and removes damaged cells from the body?
Monocyte
The illumination system of the compound microscope evenly distributes light and contains what three parts?
Internal light source, condenser, and an iris diaphragm.
What must never be used to clean the microscope because it damages the lens assembly?
Alcohol
What bacilli bacteria causes Whopping cough in a Gram-negative stain?
Bordetella pertussis
A report of transparency is of value only if the specimen is?
Fresh
What is the normal ranges of specific gravity?
1.015-1.030
What are the normal range of White Blood Cell (WBC) values in adults?
4.8-10.8 X 10*6/ml (per cubic millimeter)
What objective lens should be used for a detailed study of stained blood and bacterial smears?
Oil-immersion
Which temperature requirement category are “cold loving” bacteria that reproduces best at low temperatures (4 degrees C)?
Psychrophilic
When performing a WBC Differential, which cell has the large, scattered deep bluish-purple granules that are darker than the nucleus and may overlay the nucleus as well as the cytoplasm?
Basophil
Which type of toxin compromises part of the cell wall and is released as the bacterial cell is destroyed and this type is considered to be less potent, but may effect the patient during antibiotic therapy?
Endotoxin
The following physiology conditions can cause what condition: shortly after birth; pregnancy; appendicitis; ulcers; emotional stress; anxiety; and strenuous activity?
Leukocytosis
What is the approximate magnification of low-power objective lens?
10x
Hemoglobin determination, also known as hemoglobinometry is the measurement of what within the patient’s red blood cells?
Concentration of hemoglobin
What is the chemical preparations frequently used to detect fungi?
Potassium hydroxide (KOH)
What bacilli bacteria causes Diphtheria in a Gram-positive stain?
Corynebacterium diphtheria
Glucose and ketones both positive on the urine reagent strip may indicate?
Uncontrolled diabetes
Which part of the compound microscope framework is the platform on which a specimen is placed for examination and at the center is an aperture or hole that allows the passage of light from the condenser?
Stage
What contains a system of lenses with sufficient magnification and resolving power allowing small elements close together in a specimen to appear larger and distinctly separated?
Compound microscope
What manual method is used when blood is centrifuged to determine the hematocrit?
Microhematocrit method
What is the condition referred to when the WBC is abnormally low?
Leukopenia
Which temperature requirement category are bacteria that reproduces best at higher temperatures (42 degrees C)?
Thermophilic
Which leukocyte destroys parasites and respond immediately to allergic reactions; the cytoplasm contains numerous large reddish-orange granules?
Eosinophil
A tourniquet is normally applied before to aid in the process of venipuncture. At what point in the venipuncture procedure should the tourniquet be removed?
Once blood flows freely into tubes
Upon what activity do all urine specimens will develop a faint cloud composed of mucus, leukocytes, and epithelial cells?
Standing and cooling
What is the method used in the laboratory to identify bacteria and that can be performed in isolated duty locations or onboard Naval vessels?
Smear method
What is defined as a red cell (erythrocytes) count below normal values?
Anemia
What anticoagulant does the grey top use for chemistry (plasma samples)?
Sodium Flouride
What are the three types of urine specimens?
Random, first morning, and 24-hour
What are the urinary sediment that are formed by coagulation of albuminous material in the kidney tubules; they are cylindrical and vary in diameter, sides are parallel, and the ends are rounded?
Casts
What operational program may be used on some operational platforms to treat critical patients in a mass casualty situation when delay of blood products would cause a critical delay?
Walking Blood Bank (WBB)
What are the three physical characteristics when routine urinalysis is being evaluated?
Color, appearance, and specific gravity
Which part of the compound microscope framework holds the specimen in place and is the means by which the specimen may be moved about on the stage to view the sample?
Mechanical (movable) Stage
How far below should you “Anchor” the vein using your thumb of the free hand and slightly to the side of the intended venipuncture site, and pull the skin towards your wrist?
1-2 inches
Under what concept outlined by Occupational Safety & Health Administration (OSHA) are all human blood and certain other human body fluids are treated as if known to be infectious?
Universal Bloodborne Pathogen Precautions
How far above the intended phlebotomy site should the tourniquet be placed?
3-4 inches above the site
What is the magnification of microscopic examination of urine sediment that is fresh as possible in addition to routine chemical procedures?
40x
Fungi that appear as dense clouds are found in what part of the body or Linear rows?
Hair
Which control knob is the larger inner knob that when rotated, allows the image to appear in approximate focus?
Coarse Control Knob
What are the two principal method of obtaining blood specimens?
Capillary and venipuncture method
What is the desired urine sample volume for routine testing?
12 ml
What equipment is used to measure specific gravity?
Index refractometer
Which neutrophil sub-classification is a mature neutrophil which the nucleus is separated into two, three, four, or five lobes?
Segmented Neutrophil
The Rapid Plasma Reagin (RPR) test to screen for syphilis is best used with what type of specimen?
Serum
The following conditions may cause what: severe or advance bacterial infections (typhoid, paratyphoid, and tularemia); infections caused by virus and rickettsiae (measles, rubella, smallpox, hepatitis, psittacosis, dengue fever, and influenza); anaphylactic shock; and radiation?
Leukopenia
What type of microscope is most often used in the laboratory?
Compound microscope
What bacilli bacteria causes Brucellosis in a Gram-negative stain?
Brucella abortus
What are the two types of toxins produced as waste products of metabolism in bacterial cell?
Endotoxin and Exotoxin
Which leukocyte have cytoplasms that is clear sky blue, scanty, with few unevenly distributed, blue granules with a halo around them?
Lymphocyte
The following steps are for what urine specimen: (1) have the patient empty bladder and discard urine; (2) collect urine for 24 hours; (3) instruct patient to empty bladder at 0800 the following day and add the urine to the collection?
24-Hour Urine Specimen
Formed elements such as red and white blood cells sink to the bottom of the specimen and will be undetected by the reagent strips unless?
Urine is mixed
What does yellow or brown urine indicate the presence of?
Bile
How many weeks does Reagin usually develop after the appearance of a primary chancre?
1-4 weeks
What is the term used for bacteria that cause disease?
Pathogen
What factors affect the hemoglobin determination values?
Age, sex, disease, and altitude
The correct needle position for venipuncture is (a) what degree angle and (b) with the bevel in what position?
(a) 15-30. (b) up
What are the two lens systems on the compound microscope magnification system?
Objective and ocular lenses
What are the two parts of the compound microscope that comprise of the focusing system?
Coarse and Fine Control Knob
Casts in the urine always indicate some form of what type of disorder?
Kidney disorder
More than 3 leukocyte cells per high-power field may indicate disease where?
Urinary tract
Which color tube is the best choice for Complete Blood Count (CBC)?
Lavender top
What consists of the following tests: total Red Blood Cell (RBC) count, Hemoglobin determination (Hgb), Hematocrit (Hct), total White Blood Cell (WBC) count, and White Blood Cell Differential count?
Complete Blood Count (CBC)
The formation of hemoglobin takes place during the development of red cells (erythrocytes) located where?
Bone marrow
Who is responsible for coordinating a well-planned transfusion program?
Senior Medical Department Representative (SMDR) and embarked laboratory personnel
What is the approximate magnification for oil-immersion objective lens?
100x
What part of the compound microscope illumination system is composed of a compact lens system, located below the stage, and concentrates and focuses light from the light source on the specimen?
Condenser
Which leukocyte has an indented nucleus and abundant pale bluish-gray cytoplasm?
Monocyte
What cocci bacteria causes strep throat in a Gram-positive stain?
Streptococcus pyogenes
How is the strip held to ensure against run-over, when comparing it with the color chart?
Horizontal from the color chart
What is the primary cause of death from a Blister agent?
Massive edema or mechanical pulmonary obstruction
Which autoinjector kit is a single autoinjector that has two chambers that deliver 2.1mg of Atropine and 600mg of 2-PAM CL in a single injection?
Autoinjector Treatment Nerve Agent Antidote (ATNAA)
Which of the three types of Plague has death results from respiratory failure, circulatory collapse, and shock; and if treatment is not started within 24 hours of onset of symptoms, the disease is 100% fatal?
Pneumonic plague
Which radiation measurement device is a multi-range Radiac device that detects beta and gamma radiation and uses a Gieger Mueller ionization chamber and has two ranges for radiation levels, mR/hr and R/hr?
Radiac Set AN/PDQ-1
What are the isolation and decontamination treatment for Plague?
Respiratory isolation; get rid of patients and their clothing of fleas; cleanse patients with soap and water; use 1% bleach solution for decontamination
Which type of Bacterial Biological Agent is a disease caused by the bacteria Bacillus anthracis and is a gram-positive, encapsulated, spore forming, non-motile rod; in which spore can survive in the environment for years or decade, awaiting patient by next host?
Anthrax (Bacillus anthracis)
Which type of chemical agents is odorless, colorless liquids or vapors?
Nerve Agents
Which radiation measurement device is used to perform ground radiological surveys in vehicles or in dismounted mode by individual personnel as a handheld instrument?
Radiac Set AN/VDR-2
Which nerve agent treatment removes the agent from the enzyme acetylcholinesterase within the synaptic cleft (the space between the nerve cells) of the nervous system?
Pralidoxime Chloride (2-PAM CL)
M9 Chemical Agent Detection Paper turns what color if a nerve agent or a blister agent is present?
Pink red, reddish brown, or purple color
What are the first line antibiotic treatments for Anthrax?
Ciprofloxacin (500mg BID or 400mg Q12H IV) or Doxycycline (100mg Q12H orally or IV)
Which type of Radiation is a light, short-range particle and is actually an ejected electron? This may travel several feet in the air and can penetrate human skin to the “germinal layer”, where new skin is produced.
Beta