Chapter 16 Flashcards
Bacteriophages
Virus that infects bacteria; composed of DNA & proteins
Old problem: is _____ ______ made of _____ or _____?
genetic material, DNA, proteins
DNA structure
Double helix
DNA “backbone”
Sugar + phosphate
DNA “rungs”
Nitrogenous bases
Nucleotide diagram
P(hosphate)
S(ugar) N(itrogenous base)
Nitrogenous bases
Adenine, guanine, thymine, cytosine
DNA stands for…
Deoxyribonucleic Acid
Purine bases
Adenine, Guanine
Pyrimadine bases
Thymine, Cytosine
Adenine pairs with _____ in DNA
Thymine
Adenine pairs with _____ in RNA
Uracil
Guanine pairs with _____ in DNA
Cytosine
Type of bond between A and T
Double bond
Type of bond between G and C
Triple bond
_____ bonds between base pairs of each strand hold the molecule together like a _____
Hydrogen, zipper
Antiparallel
One strand runs from 5’ to 3’ and the other runs fom 3’ to 5’
Prokaryotic DNA is (circular/linear)
CIrcular
Eukaryotic DNA is (circular/linear)
Linear
__ chromosome(s) in prokaryotes
1
__ chromosome(s) in eukaryotes
Usually 1+
Prokaryote DNA location
Cytoplasm
Eukaryote DNA location
Nucleus
Do prokaryotes or eukaryotes have histones?
Eukaryotes
Histones
Proteins
_____ DNA in Prokaryotes
Supercoiled
Eukaryotic DNA forms _____
Chromatin
Replication model
Semiconservative
Semiconservative model def.
When DNA separates, half of DNA goes to next replication
Helicase function
Unzips/unwinds DNA at origins of replication and creates replication forks
Initiation proteins function
Separate 2 strands, which forms replication bubble
Primase
Adds RNA primer to start replication
DNA polymerase III
Adds complimentary bases to leading strand (new DNA made 5’ to 3’)
Lagging strand grows in _____ direction by the addition of _______ __________
3’ to 5’, Okazaki fragments
DNA polymerase I
Replaces RNA primers with DNA
DNA ligase
seals fragments together
Okazaki Fragments
Short segments of DNA that grow 5’ to 3’ that are added onto lagging strand
Single-strand binding protein
Binds to and stabilized single-stranded DNA until it can be used as a template
Topoisomerase
Relieves “overwinding” strain ahead of replication forks by breaking, swiveling, rejoining DNA strands
_____ ______ proofread as based added
DNA polymerases
Mismatch repair
special enzymes fix incorrect pairings
Nucleotide excision repair
Nucleases cut damaged DNA
DNA poly and ligase fill in gaps
DNA poly only adds nucleotides to ___ end
3’
Telomeres
Repeated units of short nucleotide sequences (TTAGGG) at ends of DNA
“Cap” ends of DNA to postpone erosion
Telomerase
Adds to telomeres