CHAPTER 14.1-14.2 (HALF OF FINAL) Flashcards
biotechnology
refers to the use of natural biological systems to create a product or achieve some other end desired by humans
genetic engineering
allows scientists to modify the genomes of a variety of organisms, from bacteria to plants and animals, to either improve the characteristics of the organism or make biotechnology products.
genetically modified organism (GMO)
one whose genome has been modified in some way, usually by recombinant DNA technology
transgenic organism
an example of a GMO that has had a gene from another species inserted into its genome
cloning
the production of genetically identical copies of DNA, cells, or organisms through asexual means
gene cloning
DNA cloning can be done to produce many identical copies of the same gene
Recombinant DNA (rDNA)
contains DNA from two or more different sources, such as human cell and a bacterial cell
vector
to make rDNA, a technician needs a vector (“To carry”) by which rDNA will be introduced into a host cell. EX: a plasmid
Plasmids
small accessory rings of DNA found in bacteria
restriction enzyme & DNA ligase
two enzymes needed to introduce foreign DNA into vector DNA, a restriction enzyme, which cleaves DNA and an enzyme called DNA ligase which seals DNA into an opening created by the restriction enzyme.
DNA sequencing
a procedure used to determine the order of nucleotides in a segment of DNA, often within a specific gene
gel electrophoresis
a process which separates DNA fragments according to their size, with the result of fragment sorting a pattern of distinctive brands that identify the person
DNA Fingerprinting
a technology that can identify and distinguish among individuals based on variations in their DNA
short tandem repeat (STR) sequences
short DNA sequences that are repeated many times in a row
genome editing
the targeting of specific sequences in the DNA for removal or replacement. most widely used method is called CRISPR