Chapter 13.1--Disorders of White blood cells--leukopenia Flashcards
Myeloid tissues
bone marrow and cells derived from it (red cells, platelets, granulocytes, monocytes)
Lymphoid tissues
thymus, lymph nodes, spleen
Not possible to draw neat lines bw diseases of myeloid vs. lymphoid tissues. Examples?
- bone marrow has few lymphocytes but is the source of all lymphoid progenitors and home of long lived plasma cells and memory lymphocytes
- neoplastic disorders of myeloid origebutirs cells (myeloid leukemias) originate in the bone marrow but secondarily involve spleen and lymphoid to lesser degree
Blood cell progenitors first appear when?
- third week of embryonic development in yolk sac
- cells from yolk sac are source of tissue macrophages like microglial cells in the brain and Kupffer cells
- but contribution from yolk sac is only TRANSIENT; definitive hematopoietic stem cells arise several weeks later in the mesoderm of intraembryonic aorta/gonal/mesonephros region
3rd month of embryogenesis
- HSCs migrate to the liver which becomes the chief site of blood cell formation until shortly before birth
- HSCs also take up residence in fetal placenta–HSCs harvested at birth from umbilical cord used for HSC transplant
4th month of development
- HSCs migrate to bone marrow
- by birth marrow throughout skeleton is hematopoetically active and hepatic hematopoiesis decreases, persisting only in widely scattered foci that become inactive soon after birth
Before vs. after puberty–hematopoesis
- until puberty active marrow found throughout skeleton but after puberty it becomes restricted to axial skeleton
- in normal adults only half of marrow space is hematopoetically active
The formed elements of blood–red cells, granulocytes, monocytes, platelets, and lymphocytes have a common origin from
-HSCs, pluripotent cells that sit at apex of a hierarchy of bone marrow progenitors
HSCs ultimately differentiate into what two group of cells
- HSCs give rise to early progenitor cells which have restricted differentiation to either myloid cells or lymphoid cells
- some cells are called colony forming units bc they produce colonies made of specific kinds of mature cells when grown in culture
- from progenitors–form precursors like myeloblasts, proerythroblasts and megakaryoblasts which are immediate progenitors of mature granulocytes, red cells and platelets
Two essential properties that are required for the maintenance of hematopoeisis
-pluripotency and the capacity for self renewal!
Pluripotency
- ability of a single HSC to generate all mature blood cells
- When an HSC divides, at least one daughter cell must self-renew to avoid stem cell depletion
- self-renewing divisions occur within a specialized marrow nice, in which stromal cells and secreted factors nurture and protect the HSCs
stress conditions and HSCs
- HSCs are NOT sessile; in stress conditions like anemia or inflammmation, HScs are mobilized from bone marrow and appear in peripheral blood
- HSCs used in transplantation are now mainly collected from peripheral blood of donors treated with GCSF–one of the factors that can mobilize a fraction of marrow HSCs from their stem cell niches
The marrow response to short-term physiologic needs is regulated by
- hematopoetic growth factors through effects on the committed progenitors
- since mature blood elements are terminally differentiated cells with finite lifespans, their numbers must be constantly replenished
Multipotent progenitors
- more proliferative than HSCs but lesser capacity for self renewal
- Division of multipotent progenitors gives rise to at least one daughter cell that leaves the stem cell pool and begins to differentiate
- Once past this threshold, the newly committed cells lose capacity for self renewal and commence an inexorable journey down a road that leads to terminal differentiation and death but as these progenitors differentiate, they also begin to proliferate more rapidly in response to growth factors, expanding their numbers
stem cell factor aka KIT ligand and FLT3-ligand act through?
-receptors that are expressed on very early committed progenitors
erythropoetin, GM-CSF, G-CSF and thrombopoetin act through
-receptors that are only expressed committed progenitors with more restricted differentiation potentials
What tunes the marrow output allowing numbers of formed blood elements (red cells, white cells and platelets) to be maintained within appropriate ranges?
-Feedback loops involving lineage-specific growth factors
Many diseases do what to blood cells?
- alter the production of blood cells
- marrow is ultimate source of most cells of innate and adaptive immune system and responds to infectious or inflammatory challenges by increasing its output of granulocytes under direction of specific growth factors and cytokines
- In contrast, many other disorders are associated with defects in hematopoeisis that lead to deficiencies of one or more types of blood cells
Diseases that interfere with production of blood cells by the marrow
- primary tumors of hematopoetic cells–most important
- also–genetic diseases, chronic inflammation
Tumors of hematopoetic origin are often associated with mutations that
- block progenitor cell maturation or abrogate their growth factor dependence
- net effect is–unregulated clonal expansion of hematopoetic elements which replace normal marrow progenitors and spread to other hematopoeietic tissues
- sometimes these tumors originate from transformed HSC that retain ability to differentation into multiple lineages whereas other times origin is more differentiated progenitor that has acquired an abnormal capacity for self renewal